ME3

Protein identityUniProt · HPA

UniProt accession

Q16798

Gene

ME3

Ensembl

ENSG00000151376

Chromosome

11

Canonical length

604 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

604 residues, UniProt reviewed canonical sequence.

>Q16798|ME3
     1  MGAALGTGTR LAPWPGRACG ALPRWTPTAP AQGCHSKPGP ARPVPLKKRG YDVTRNPHLN
    61  KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYERQ QSDLDKYIIL MTLQDRNEKL
   121  FYRVLTSDVE KFMPIVYTPT VGLACQHYGL TFRRPRGLFI TIHDKGHLAT MLNSWPEDNI
   181  KAVVVTDGER ILGLGDLGCY GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD
   241  PLYIGLKHQR VHGKAYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM
   301  FNDDIQGTAS VAVAGILAAL RITKNKLSNH VFVFQGAGEA AMGIAHLLVM ALEKEGVPKA
   361  EATRKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE EVVRLVKPTA IIGVAAIAGA
   421  FTEQILRDMA SFHERPIIFA LSNPTSKAEC TAEKCYRVTE GRGIFASGSP FKSVTLEDGK
   481  TFIPGQGNNA YVFPGVALGV IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST
   541  IRDVSLRIAI KVLDYAYKHN LASYYPEPKD KEAFVRSLVY TPDYDSFTLD SYTWPKEAMN
   601  VQTV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ME3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.25

Highest tissue expression

29 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.07

gnomAD pLI

0

gnomAD missense Z

1.33

DepMap mean gene effect

0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• malate metabolic process
• pyruvate biosynthetic process

Molecular functions

• malate dehydrogenase (decarboxylating) (NADP+) activity
• malic enzyme activity
• metal ion binding
• NAD binding
• NADP+ binding
• oxaloacetate decarboxylase activity

Cellular components

• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Malic oxidoreductase
• Malic enzyme, N-terminal domain
• Malic enzyme, NAD-binding
• Malic enzyme, conserved site
• NAD(P)-binding domain superfamily
• Malic enzyme, N-terminal domain superfamily
• Aminoacid dehydrogenase-like, N-terminal domain superfamily
• Malic enzyme, N-terminal domain
• Malic enzyme, NAD binding domain

KeywordsUniProt

• Metal-binding
• Mitochondrion
• NADP
• Oxidoreductase
• Phosphoprotein
• Transit peptide