ME1

Protein identityUniProt · HPA

UniProt accession

P48163

Gene

ME1

Ensembl

ENSG00000065833

Chromosome

6

Canonical length

572 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Plasma membrane,Cytosol

Quaternary structure

Homotetramer

OverviewNCBI Gene

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

572 residues, UniProt reviewed canonical sequence.

>P48163|ME1
     1  MEPEAPRRRH THQRGYLLTR NPHLNKDLAF TLEERQQLNI HGLLPPSFNS QEIQVLRVVK
    61  NFEHLNSDFD RYLLLMDLQD RNEKLFYRVL TSDIEKFMPI VYTPTVGLAC QQYSLVFRKP
   121  RGLFITIHDR GHIASVLNAW PEDVIKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC
   181  GGMNPQECLP VILDVGTENE ELLKDPLYIG LRQRRVRGSE YDDFLDEFME AVSSKYGMNC
   241  LIQFEDFANV NAFRLLNKYR NQYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTILFQ
   301  GAGEAALGIA HLIVMALEKE GLPKEKAIKK IWLVDSKGLI VKGRASLTQE KEKFAHEHEE
   361  MKNLEAIVQE IKPTALIGVA AIGGAFSEQI LKDMAAFNER PIIFALSNPT SKAECSAEQC
   421  YKITKGRAIF ASGSPFDPVT LPNGQTLYPG QGNNSYVFPG VALGVVACGL RQITDNIFLT
   481  TAEVIAQQVS DKHLEEGRLY PPLNTIRDVS LKIAEKIVKD AYQEKTATVY PEPQNKEAFV
   541  RSQMYSTDYD QILPDCYSWP EEVQKIQTKV DQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ME1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.22

Highest tissue expression

42 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ME1.

Disease | ImmuneIEDB

Conditions an epitope on ME1 was assayed in.

• lung non-small cell carcinoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.17

gnomAD pLI

0

gnomAD missense Z

-0.29

DepMap mean gene effect

-0.17

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• carbohydrate metabolic process
• malate metabolic process
• NAD+ metabolic process
• NADP+ metabolic process
• nucleotide biosynthetic process
• protein homotetramerization
• regulation of NADP metabolic process
• response to carbohydrate
• response to hormone

Molecular functions

• ADP binding
• electron transfer activity
• identical protein binding
• magnesium ion binding
• malate dehydrogenase (decarboxylating) (NADP+) activity
• malic enzyme activity
• manganese ion binding
• NAD binding
• NADP binding
• oxaloacetate decarboxylase activity

Cellular components

• cytoplasm
• cytosol
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Malic oxidoreductase
• Malic enzyme, N-terminal domain
• Malic enzyme, NAD-binding
• Malic enzyme, conserved site
• NAD(P)-binding domain superfamily
• Malic enzyme, N-terminal domain superfamily
• Aminoacid dehydrogenase-like, N-terminal domain superfamily
• Malic enzyme, N-terminal domain
• Malic enzyme, NAD binding domain

KeywordsUniProt

• Acetylation
• Cytoplasm
• Metal-binding
• NADP
• Oxidoreductase
• Phosphoprotein