Seroatlas · Human Serome Atlas

MDH1B

Putative malate dehydrogenase 1B

Also known as: FLJ25341, MDH1B_HUMAN, RP11-95H11

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5I0G3
Gene: MDH1B
Ensembl: ENSG00000138400
Chromosome: 2
Canonical length: 518 aa
Protein class: Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable L-malate dehydrogenase (NAD+) activity. Predicted to be involved in NADH metabolic process; dicarboxylic acid metabolic process; and tricarboxylic acid cycle. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

518 residues, UniProt reviewed canonical sequence.

>Q5I0G3|MDH1B
     1  MAKFVIAGRA DCPYYAKTEL VADYLQKNLP DFRIHKITQR PEVWEDWLKD VCEKNKWSHK
    61  NSPIIWRELL DRGGKGLLLG GYNEFLEHAQ LYYDVTSSMT TELMMVIAQE NLGAHIEKEQ
   121  EEEALKTCIN PLQVWITSAS APACYNLIPI LTSGEVFGMH TEISITLFDN KQAEEHLKSL
   181  VVETQDLASP VLRSVSICTK VEEAFRQAHV IVVLDDSTNK EVFTLEDCLR SRVPLCRLYG
   241  YLIEKNAHES VRVIVGGRTF VNLKTVLLMR YAPRIAHNII AVALGVEGEA KAILARKLKT
   301  APSYIKDVII WGNISGNNYV DLRKTRVYRY ESAIWGPLHY SRPVLNLIFD SEWVKREFVA
   361  ILKNLTTTGR QFGGILAAHS IATTLKYWYH GSPPGEIVSL GILSEGQFGI PKGIVFSMPV
   421  KFENGTWVVL TDLKDVEISE QIMTRMTSDL IQEKLVALGD KIHFQPYQSG HKDLVPDEEK
   481  NLAMSDAAEF PNQIPQTTFE KPQSLEFLNE FEGKTVES

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MDH1B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

testis: 23 nTPM
fallopian tube: 21 nTPM
choroid plexus: 20 nTPM
pituitary gland: 9.1 nTPM
retina: 7.9 nTPM
thyroid gland: 4.7 nTPM

Single-cell type

late primary spermatocytes: 348 nCPM
early spermatids: 159 nCPM
respiratory ciliated cells: 127 nCPM
fallopian tube ciliated cells: 117 nCPM
ependymal cells: 79 nCPM
endometrial ciliated cells: 64 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

choroid plexus: 32 nTPM
midbrain: 10 nTPM
medulla oblongata: 7.8 nTPM
spinal cord: 6 nTPM
white matter: 5.1 nTPM
hypothalamus: 4.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.18
gnomAD pLI: 0
gnomAD missense Z: 0.45
DepMap mean gene effect: -0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

L-malate dehydrogenase (NAD+) activity

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MDH1B as an antibody target. Whether an autoantibody or antibody against MDH1B could matter depends on whether native MDH1B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MDH1B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MDH1B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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