MARCHF7

Protein identityUniProt · HPA

UniProt accession

Q9H992

Gene

MARCHF7

Ensembl

ENSG00000136536

Chromosome

2

Canonical length

704 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Plasma membrane,Basal body,Cytosol

OverviewNCBI Gene

MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Mar 2010]

Canonical amino-acid sequenceUniProt

704 residues, UniProt reviewed canonical sequence.

>Q9H992|MARCHF7
     1  MESKPSRIPR RISVQPSSSL SARMMSGSRG SSLNDTYHSR DSSFRLDSEY QSTSASASAS
    61  PFQSAWYSES EITQGARSRS QNQQRDHDSK RPKLSCTNCT TSAGRNVGNG LNTLSDSSWR
   121  HSQVPRSSSM VLGSFGTDLM RERRDLERRT DSSISNLMDY SHRSGDFTTS SYVQDRVPSY
   181  SQGARPKENS MSTLQLNTSS TNHQLPSEHQ TILSSRDSRN SLRSNFSSRE SESSRSNTQP
   241  GFSYSSSRDE APIISNSERV VSSQRPFQES SDNEGRRTTR RLLSRIASSM SSTFFSRRSS
   301  QDSLNTRSLN SENSYVSPRI LTASQSRSNV PSASEVPDNR ASEASQGFRF LRRRWGLSSL
   361  SHNHSSESDS ENFNQESEGR NTGPWLSSSL RNRCTPLFSR RRREGRDESS RIPTSDTSSR
   421  SHIFRRESNE VVHLEAQNDP LGAAANRPQA SAASSSATTG GSTSDSAQGG RNTGISGILP
   481  GSLFRFAVPP ALGSNLTDNV MITVDIIPSG WNSADGKSDK TKSAPSRDPE RLQKIKESLL
   541  LEDSEEEEGD LCRICQMAAA SSSNLLIEPC KCTGSLQYVH QDCMKKWLQA KINSGSSLEA
   601  VTTCELCKEK LELNLEDFDI HELHRAHANE QAEYEFISSG LYLVVLLHLC EQSFSDMMGN
   661  TNEPSTRVRF INLARTLQAH MEDLETSEDD SEEDGDHNRT FDIA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF7 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.64

Highest tissue expression

63 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.38

gnomAD pLI

0.66

DepMap mean gene effect

-0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of cilium assembly
• negative regulation of DNA damage response, signal transduction by p53 class mediator
• negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
• negative regulation of proteasomal protein catabolic process
• negative regulation of protein autoubiquitination
• negative regulation of T cell proliferation
• positive regulation of cell population proliferation
• positive regulation of protein polyubiquitination
• protein autoubiquitination
• protein monoubiquitination
• protein stabilization
• regulation of tolerance induction

Molecular functions

• MDM2/MDM4 family protein binding
• ubiquitin binding
• ubiquitin conjugating enzyme binding
• ubiquitin protein ligase activity
• zinc ion binding

Cellular components

• centrosome
• ciliary basal body
• cytosol
• nucleus
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Zinc finger, RING-CH-type
• Zinc finger, RING/FYVE/PHD-type
• RING-CH-type E3 ubiquitin-protein ligase
• RING-variant domain

KeywordsUniProt

• Acetylation
• Cytoplasm
• Metal-binding
• Phosphoprotein
• Transferase
• Ubl conjugation pathway
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of MARCHF7 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MAPRE1
• TGFBR2
• UBE2K