Seroatlas · Human Serome Atlas

KNTC1

Kinetochore-associated protein 1

Also known as: KIAA0166, KNTC1_HUMAN, ROD

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P50748
Gene: KNTC1
Ensembl: ENSG00000184445
Chromosome: 12
Canonical length: 2209 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

2209 residues, UniProt reviewed canonical sequence.

>P50748|KNTC1
     1  MWNDIELLTN DDTGSGYLSV GSRKEHGTAL YQVDLLVKIS SEKASLNPKI QACSLSDGFI
    61  IVADQSVILL DSICRSLQLH LVFDTEVDVV GLCQEGKFLL VGERSGNLHL IHVTSKQTLL
   121  TNAFVQKAND ENRRTYQNLV IEKDGSNEGT YYMLLLTYSG FFCITNLQLL KIQQAIENVD
   181  FSTAKKLQGQ IKSSFISTEN YHTLGCLSLV AGDLASEVPV IIGGTGNCAF SKWEPDSSKK
   241  GMTVKNLIDA EIIKGAKKFQ LIDNLLFVLD TDNVLSLWDI YTLTPVWNWP SLHVEEFLLT
   301  TEADSPSSVT WQGITNLKLI ALTASANKKM KNLMVYSLPT MEILYSLEVS SVSSLVQTGI
   361  STDTIYLLEG VCKNDPKLSE DSVSVLVLRC LTEALPENRL SRLLHKHRFA EAESFAIQFG
   421  LDVELVYKVK SNHILEKLAL SSVDASEQTE WQQLVDDAKE NLHKIQDDEF VVNYCLKAQW
   481  ITYETTQEML NYAKTRLLKK EDKTALIYSD GLKEVLRAHA KLTTFYGAFG PEKFSGSSWI
   541  EFLNNEDDLK DIFLQLKEGN LVCAQYLWLR HRANFESRFD VKMLESLLNS MSASVSLQKL
   601  CPWFKNDVIP FVRRTVPEGQ IILAKWLEQA ARNLELTDKA NWPENGLQLA EIFFTAEKTD
   661  ELGLASSWHW ISLKDYQNTE EVCQLRTLVN NLRELITLHR KYNCKLALSD FEKENTTTIV
   721  FRMFDKVLAP ELIPSILEKF IRVYMREHDL QEEELLLLYI EDLLNRCSSK STSLFETAWE
   781  AKAMAVIACL SDTDLIFDAV LKIMYAAVVP WSAAVEQLVK QHLEMDHPKV KLLQESYKLM
   841  EMKKLLRGYG IREVNLLNKE IMRVVRYILK QDVPSSLEDA LKVAQAFMLS DDEIYSLRII
   901  DLIDREQGED CLLLLKSLPP AEAEKTAERV IIWARLALQE EPDHSKEGKA WRMSVAKTSV
   961  DILKILCDIQ KDNLQKKDEC EEMLKLFKEV ASLQENFEVF LSFEDYSNSS LVADLREQHI
  1021  KAHEVAQAKH KPGSTPEPIA AEVRSPSMES KLHRQALALQ MSKQELEAEL TLRALKDGNI
  1081  KTALKKCSDL FKYHCNADTG KLLFLTCQKL CQMLADNVPV TVPVGLNLPS MIHDLASQAA
  1141  TICSPDFLLD ALELCKHTLM AVELSRQCQM DDCGILMKAS FGTHKDPYEE WSYSDFFSED
  1201  GIVLESQMVL PVIYELISSL VPLAESKRYP LESTSLPYCS LNEGDGLVLP VINSISALLQ
  1261  NLQESSQWEL ALRFVVGSFG TCLQHSVSNF MNATLSEKLF GETTLVKSRH VVMELKEKAV
  1321  IFIRENATTL LHKVFNCRLV DLDLALGYCT LLPQKDVFEN LWKLIDKAWQ NYDKILAISL
  1381  VGSELASLYQ EIEMGLKFRE LSTDAQWGIR LGKLGISFQP VFRQHFLTKK DLIKALVENI
  1441  DMDTSLILEY CSTFQLDCDA VLQLFIETLL HNTNAGQGQG DASMDSAKRR HPKLLAKALE
  1501  MVPLLTSTKD LVISLSGILH KLDPYDYEMI EVVLKVIERA DEKITNININ QALSILKHLK
  1561  SYRRISPPVD LEYQYMLEHV ITLPSAAQTR LPFHLIFFGT AQNFWKILST ELSEESFPTL
  1621  LLISKLMKFS LDTLYVSTAK HVFEKKLKPK LLKLTQAKSS TLINKEITKI TQTIESCLLS
  1681  IVNPEWAVAI AISLAQDIPE GSFKISALKF CLYLAERWLQ NIPSQDEKRE KAEALLKKLH
  1741  IQYRRSGTEA VLIAHKLNTE EYLRVIGKPA HLIVSLYEHP SINQRIQNSS GTDYPDIHAA
  1801  AKEIAEVNEI NLEKVWDMLL EKWLCPSTKP GEKPSELFEL QEDEALRRVQ YLLLSRPIDY
  1861  SSRMLFVFAT STTTTLGMHQ LTFAHRTRAL QCLFYLADKE TIESLFKKPI EEVKSYLRCI
  1921  TFLASFETLN IPITYELFCS SPKEGMIKGL WKNHSHESMA VRLVTELCLE YKIYDLQLWN
  1981  GLLQKLLGFN MIPYLRKVLK AISSIHSLWQ VPYFSKAWQR VIQIPLLSAS CPLSPDQLSD
  2041  CSESLIAVLE CPVSGDLDLI GVARQYIQLE LPAFALACLM LMPHSEKRHQ QIKNFLGSCD
  2101  PQVILKQLEE HMNTGQLAGF SHQIRSLILN NIINKKEFGI LAKTKYFQML KMHAMNTNNI
  2161  TELVNYLAND LSLDEASVLI TEYSKHCGKP VPPDTAPCEI LKMFLSGLS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against KNTC1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 40 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 40 nTPM
testis: 27 nTPM
spleen: 18 nTPM
thymus: 17 nTPM
tonsil: 16 nTPM
lymph node: 15 nTPM

Single-cell type

erythrocyte progenitors: 105 nCPM
monocyte progenitors: 102 nCPM
early primary spermatocytes: 96 nCPM
differentiating spermatogonia: 78 nCPM
megakaryocyte progenitors: 74 nCPM
plasma cells: 73 nCPM

Immune cell

basophil: 2.4 nTPM
eosinophil: 1.8 nTPM
memory B-cell: 1.6 nTPM
neutrophil: 1.6 nTPM
memory CD8 T-cell: 1.3 nTPM
memory CD4 T-cell: 1.1 nTPM

Brain region

white matter: 12 nTPM
cerebellum: 12 nTPM
thalamus: 11 nTPM
cerebral cortex: 11 nTPM
pons: 10 nTPM
medulla oblongata: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.83
gnomAD pLI: 0
gnomAD missense Z: 0.91
DepMap mean gene effect: -0.2
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

cell division
mitotic sister chromatid segregation
mitotic spindle assembly checkpoint signaling
protein-containing complex assembly
regulation of attachment of spindle microtubules to kinetochore
regulation of exit from mitosis
protein localization to kinetochore involved in kinetochore assembly

Molecular functions

small GTPase binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

WD40-repeat-containing domain superfamily
RZZ complex, subunit KNTC1/ROD, C-terminal
Kinetochore-associated protein 1
KNTC1, N-terminal
KNTC1, first ARM-repeats domain
KNTC1, second ARM-repeats domain
KNTC1, third ARM-repeats domain
Rough deal protein C-terminal region
KNTC1, N-terminal
KNTC1 third ARM-repeats domain
KNTC1 second ARM-repeats domain
KNTC1 first ARM-repeats domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of KNTC1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads KNTC1 as an antibody target. Whether an autoantibody or antibody against KNTC1 could matter depends on whether native KNTC1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

KNTC1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label KNTC1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...