Seroatlas · Human Serome Atlas

ZWILCH

Protein zwilch homolog

Also known as: FLJ10036, KNTC1AP, ZWILC_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H900
Gene: ZWILCH
Ensembl: ENSG00000174442
Chromosome: 15
Canonical length: 591 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Involved in mitotic spindle assembly checkpoint signaling and protein localization to kinetochore. Located in kinetochore. Part of RZZ complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

591 residues, UniProt reviewed canonical sequence.

>Q9H900|ZWILCH
     1  MWERLNCAAE DFYSRLLQKF NEEKKGIRKD PFLYEADVQV QLISKGQPNP LKNILNENDI
    61  VFIVEKVPLE KEETSHIEEL QSEETAISDF STGENVGPLA LPVGKARQLI GLYTMAHNPN
   121  MTHLKINLPV TALPPLWVRC DSSDPEGTCW LGAELITTNN SITGIVLYVV SCKADKNYSV
   181  NLENLKNLHK KRHHLSTVTS KGFAQYELFK SSALDDTITA SQTAIALDIS WSPVDEILQI
   241  PPLSSTATLN IKVESGEPRG PLNHLYRELK FLLVLADGLR TGVTEWLEPL EAKSAVELVQ
   301  EFLNDLNKLD GFGDSTKKDT EVETLKHDTA AVDRSVKRLF KVRSDLDFAE QLWCKMSSSV
   361  ISYQDLVKCF TLIIQSLQRG DIQPWLHSGS NSLLSKLIHQ SYHGTMDTVS LSGTIPVQML
   421  LEIGLDKLKK DYISFFIGQE LASLNHLEYF IAPSVDIQEQ VYRVQKLHHI LEILVSCMPF
   481  IKSQHELLFS LTQICIKYYK QNPLDEQHIF QLPVRPTAVK NLYQSEKPQK WRVEIYSGQK
   541  KIKTVWQLSD SSPIDHLNFH KPDFSELTLN GSLEERIFFT NMVTCSQVHF K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZWILCH can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 50 nTPM

Expression across tissuesHPA

Tissue

testis: 50 nTPM
thymus: 13 nTPM
tonsil: 13 nTPM
lymph node: 11 nTPM
bone marrow: 9.8 nTPM
rectum: 9.2 nTPM

Single-cell type

late primary spermatocytes: 206 nCPM
late spermatids: 117 nCPM
early spermatids: 116 nCPM
early primary spermatocytes: 114 nCPM
differentiating spermatogonia: 64 nCPM
monocyte progenitors: 50 nCPM

Immune cell

non-classical monocyte: 5.4 nTPM
NK-cell: 5 nTPM
basophil: 4.5 nTPM
T-reg: 4.5 nTPM
memory B-cell: 4.4 nTPM
gdT-cell: 4.3 nTPM

Brain region

choroid plexus: 9.7 nTPM
white matter: 9.2 nTPM
medulla oblongata: 8.2 nTPM
hypothalamus: 7.4 nTPM
spinal cord: 7.4 nTPM
cerebral cortex: 7.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.02
gnomAD pLI: 0
gnomAD missense Z: -0.39
DepMap mean gene effect: -0.23
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

RZZ complex, subunit Zwilch
RZZ complex, subunit zwilch

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZWILCH in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

KNTC1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZWILCH as an antibody target. Whether an autoantibody or antibody against ZWILCH could matter depends on whether native ZWILCH is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZWILCH is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZWILCH as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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