Seroatlas · Human Serome Atlas

HSD3B1

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Also known as: 3BHS1_HUMAN, HSD3B, HSDB3, SDR11E1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P14060
Gene
HSD3B1
Ensembl
ENSG00000203857
Chromosome
1
Canonical length
373 aa
Protein class
Enzymes, FDA approved drug targets, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Nucleoli,Endoplasmic reticulum,Microtubules,Cytokinetic bridge,Primary cilium,Primary cilium transition zone

OverviewNCBI Gene

The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016]

Canonical amino-acid sequenceUniProt

373 residues, UniProt reviewed canonical sequence.

>P14060|HSD3B1
     1  MTGWSCLVTG AGGFLGQRII RLLVKEKELK EIRVLDKAFG PELREEFSKL QNKTKLTVLE
    61  GDILDEPFLK RACQDVSVII HTACIIDVFG VTHRESIMNV NVKGTQLLLE ACVQASVPVF
   121  IYTSSIEVAG PNSYKEIIQN GHEEEPLENT WPAPYPHSKK LAEKAVLAAN GWNLKNGGTL
   181  YTCALRPMYI YGEGSRFLSA SINEALNNNG ILSSVGKFST VNPVYVGNVA WAHILALRAL
   241  QDPKKAPSIR GQFYYISDDT PHQSYDNLNY TLSKEFGLRL DSRWSFPLSL MYWIGFLLEI
   301  VSFLLRPIYT YRPPFNRHIV TLSNSVFTFS YKKAQRDLAY KPLYSWEEAK QKTVEWVGSL
   361  VDRHKETLKS KTQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HSD3B1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.23
Highest tissue expression
73 nTPM

Expression across tissuesHPA

Tissue

  • placenta: 73 nTPM
  • small intestine: 8.3 nTPM
  • skin: 4 nTPM
  • breast: 1.4 nTPM
  • adrenal gland: 1.1 nTPM
  • liver: 1.1 nTPM

Single-cell type

  • syncytiotrophoblasts: 782 nCPM
  • extravillous trophoblasts: 267 nCPM
  • migrating cytotrophoblasts: 142 nCPM
  • enterocytes: 45 nCPM
  • cytotrophoblasts: 9.6 nCPM
  • epicardial cells: 6.8 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • cerebral cortex: 6 nTPM
  • white matter: 3.5 nTPM
  • amygdala: 3.4 nTPM
  • pons: 2.5 nTPM
  • thalamus: 2.2 nTPM
  • basal ganglia: 2.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.44
gnomAD pLI
0
gnomAD missense Z
-1.01
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HSD3B1 as an antibody target. Whether an autoantibody or antibody against HSD3B1 could matter depends on whether native HSD3B1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HSD3B1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HSD3B1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/HSD3B1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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