Seroatlas · Human Serome Atlas

HROB

Homologous recombination OB-fold protein

Also known as: C17orf53, HROB_HUMAN, MCM8IP, MGC3130

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N3J3
Gene
HROB
Ensembl
ENSG00000125319
Chromosome
17
Canonical length
647 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to enable single-stranded DNA binding activity. Involved in DNA synthesis involved in DNA repair and interstrand cross-link repair. Located in site of DNA damage. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

647 residues, UniProt reviewed canonical sequence.

>Q8N3J3|HROB
     1  MACSLQKLFA VEEEFEDEDF LSAVEDAENR FTGSLPVNAG RLRPVSSRPQ ETVQAQSSRL
    61  LLLHPTAPSE ALGLPDLDLC LPASSTPSAD SRPSCIGAAP LRPVSTSSSW IGNQRRVTVT
   121  EVLRETARPQ SSALHPLLTF ESQQQQVGGF EGPEQDEFDK VLASMELEEP GMELECGVSS
   181  EAIPILPAQQ REGSVLAKKA RVVDLSGSCQ KGPVPAIHKA GIMSAQDESL DPVIQCRTPR
   241  PPLRPGAVGH LPVPTALTVP TQQLHWEVCP QRSPVQALQP LQAARGTIQS SPQNRFPCQP
   301  FQSPSSWLSG KAHLPRPRTP NSSCSTPSRT SSGLFPRIPL QPQAPVSSIG SPVGTPKGPQ
   361  GALQTPIVTN HLVQLVTAAS RTPQQPTHPS TRAKTRRFPG PAGILPHQQS GRSLEDIMVS
   421  APQTPTHGAL AKFQTEIVAS SQASVEEDFG RGPWLTMKST LGLDERDPSC FLCTYSIVMV
   481  LRKQAALKQL PRNKVPNMAV MIKSLTRSTM DASVVFKDPT GEMQGTVHRL LLETCQNELK
   541  PGSVLLLKQI GVFSPSLRNH YLNVTPNNLV HIYSPDSGDG SFLKPSQPFP KDSGSFQHDV
   601  AAKPEEGFRT AQNLEAEASP EEELPEADDL DGLLSELPED FFCGTSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HROB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.63
Highest tissue expression
11 nTPM

Expression across tissuesHPA

Tissue

  • testis: 11 nTPM
  • bone marrow: 8.8 nTPM
  • esophagus: 2.6 nTPM
  • thymus: 2.2 nTPM
  • tonsil: 1.7 nTPM
  • skin: 1.6 nTPM

Single-cell type

  • oocytes: 37 nCPM
  • epicardial cells: 25 nCPM
  • undifferentiated spermatogonia: 24 nCPM
  • differentiating spermatogonia: 17 nCPM
  • extravillous trophoblasts: 15 nCPM
  • early primary spermatocytes: 15 nCPM

Immune cell

  • plasmacytoid DC: 0.9 nTPM
  • T-reg: 0.4 nTPM
  • naive B-cell: 0.3 nTPM
  • classical monocyte: 0.2 nTPM
  • eosinophil: 0.2 nTPM
  • gdT-cell: 0.2 nTPM

Brain region

  • cerebellum: 1.7 nTPM
  • pons: 1.5 nTPM
  • medulla oblongata: 1.3 nTPM
  • midbrain: 1.3 nTPM
  • white matter: 1.3 nTPM
  • cerebral cortex: 1.2 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about HROB.

Disease | AllUniProt

Conditions HROB is implicated in, by any mechanism.

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 20 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.05
gnomAD pLI
0
DepMap mean gene effect
-0.23
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Homologous recombination OB-fold protein
  • Homologous recombination OB-fold protein, OB-fold domain
  • Homologous recombination OB-fold protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of HROB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HROB as an antibody target. Whether an autoantibody or antibody against HROB could matter depends on whether native HROB is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HROB is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HROB as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/HROB. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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