Seroatlas · Human Serome Atlas

HERC3

Probable E3 ubiquitin-protein ligase HERC3

Also known as: HERC3_HUMAN, KIAA0032

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q15034
Gene: HERC3
Ensembl: ENSG00000138641
Chromosome: 4
Canonical length: 1050 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]

Canonical amino-acid sequenceUniProt

1050 residues, UniProt reviewed canonical sequence.

>Q15034|HERC3
     1  MLCWGYWSLG QPGISTNLQG IVAEPQVCGF ISDRSVKEVA CGGNHSVFLL EDGEVYTCGL
    61  NTKGQLGHER EGNKPEQIGA LADQHIIHVA CGESHSLALS DRGQLFSWGA GSDGQLGLMT
   121  TEDSVAVPRL IQKLNQQTIL QVSCGNWHCL ALAADGQFFT WGKNSHGQLG LGKEFPSQAS
   181  PQRVRSLEGI PLAQVAAGGA HSFALSLSGA VFGWGMNNAG QLGLSDEKDR ESPCHVKLLR
   241  TQKVVYISCG EEHTAVLTKS GGVFTFGAGS CGQLGHDSMN DEVNPRRVLE LMGSEVTQIA
   301  CGRQHTLAFV PSSGLIYAFG CGARGQLGTG HTCNVKCPSP VKGYWAAHSG QLSARADRFK
   361  YHIVKQIFSG GDQTFVLCSK YENYSPAVDF RTMNQAHYTS LINDETIAVW RQKLSEHNNA
   421  NTINGVVQIL SSAACWNGSF LEKKIDEHFK TSPKIPGIDL NSTRVLFEKL MNSQHSMILE
   481  QILNSFESCL IPQLSSSPPD VEAMRIYLIL PEFPLLQDSK YYITLTIPLA MAILRLDTNP
   541  SKVLDNWWSQ VCPKYFMKLV NLYKGAVLYL LRGRKTFLIP VLFNNYITAA LKLLEKLYKV
   601  NLKVKHVEYD TFYIPEISNL VDIQEDYLMW FLHQAGMKAR PSIIQDTVTL CSYPFIFDAQ
   661  AKTKMLQTDA ELQMQVAVNG ANLQNVFMLL TLEPLLARSP FLVLHVRRNN LVGDALRELS
   721  IHSDIDLKKP LKVIFDGEEA VDAGGVTKEF FLLLLKELLN PIYGMFTYYQ DSNLLWFSDT
   781  CFVEHNWFHL IGITCGLAIY NSTVVDLHFP LALYKKLLNV KPGLEDLKEL SPTEGRSLQE
   841  LLDYPGEDVE ETFCLNFTIC RESYGVIEQK KLIPGGDNVT VCKDNRQEFV DAYVNYVFQI
   901  SVHEWYTAFS SGFLKVCGGK VLELFQPSEL RAMMVGNSNY NWEELEETAI YKGDYSATHP
   961  TVKLFWETFH EFPLEKKKKF LLFLTGSDRI PIYGMASLQI VIQSTASGEE YLPVAHTCYN
  1021  LLDLPKYSSK EILSARLTQA LDNYEGFSLA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HERC3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.23
Highest tissue expression: 106 nTPM

Expression across tissuesHPA

Tissue

retina: 106 nTPM
cerebellum: 25 nTPM
bone marrow: 23 nTPM
tongue: 23 nTPM
heart muscle: 21 nTPM
parathyroid gland: 20 nTPM

Single-cell type

microglia: 184 nCPM
choroid plexus epithelial cells: 115 nCPM
oligodendrocytes: 113 nCPM
brain inhibitory neurons: 101 nCPM
brain excitatory neurons: 95 nCPM
renal connecting tubule cells: 91 nCPM

Immune cell

neutrophil: 16 nTPM
non-classical monocyte: 9.3 nTPM
T-reg: 8.1 nTPM
basophil: 7.8 nTPM
eosinophil: 7.3 nTPM
naive CD4 T-cell: 7 nTPM

Brain region

cerebellum: 69 nTPM
white matter: 52 nTPM
pons: 48 nTPM
basal ganglia: 46 nTPM
cerebral cortex: 44 nTPM
medulla oblongata: 42 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.39
gnomAD pLI: 0.07
gnomAD missense Z: 3.21
DepMap mean gene effect: -0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

ubiquitin protein ligase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of HERC3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

RPL23A

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HERC3 as an antibody target. Whether an autoantibody or antibody against HERC3 could matter depends on whether native HERC3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HERC3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HERC3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...