HELT

Protein identityUniProt · HPA

UniProt accession

A6NFD8

Gene

HELT

Ensembl

ENSG00000187821

Chromosome

4

Canonical length

242 aa

Protein class

Predicted intracellular proteins, Transcription factors

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of neurogenesis. Predicted to act upstream of or within several processes, including nervous system development; regulation of transcription by RNA polymerase II; and suckling behavior. Predicted to be located in chromatin. Predicted to be part of transcription regulator complex. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

242 residues, UniProt reviewed canonical sequence.

>A6NFD8|HELT
     1  MSDKLKERKR TPVSHKVIEK RRRDRINRCL NELGKTVPMA LAKQSSGKLE KAEILEMTVQ
    61  YLRALHSADF PRGREKAELL AEFANYFHYG YHECMKNLVH YLTTVERMET KDTKYARILA
   121  FLQSKARLGA EPAFPPLGSL PEPDFSYQLH PAGPEFAGHS PGEAAVFPQG SGAGPFPWPP
   181  GAARSPALPY LPSAPVPLAS PAQQHSPFLT PVQGLDRHYL NLIGHAHPNA LNLHTPQHPP
   241  VL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HELT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.57

Highest tissue expression

1.6 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.18

gnomAD pLI

0

gnomAD missense Z

-0.23

DepMap mean gene effect

0.09

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• anterior/posterior pattern specification
• central nervous system development
• multicellular organism growth
• positive regulation of transcription by RNA polymerase II
• post-embryonic development
• regulation of neurogenesis
• regulation of transcription by RNA polymerase II
• suckling behavior
• transcription by RNA polymerase II
• GABAergic neuron differentiation in basal ganglia

Molecular functions

• DNA-binding transcription factor activity, RNA polymerase II-specific
• DNA-binding transcription repressor activity, RNA polymerase II-specific
• identical protein binding
• protein dimerization activity
• RNA polymerase II cis-regulatory region sequence-specific DNA binding

Cellular components

• chromatin
• nucleus
• transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• Orange domain
• Myc-type, basic helix-loop-helix (bHLH) domain
• Helix-loop-helix DNA-binding domain superfamily
• Hairy and Enhancer of Split/HEY-related
• Helix-loop-helix DNA-binding domain
• Hairy Orange

KeywordsUniProt

• Acetylation
• DNA-binding
• Nucleus
• Repressor
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of HELT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HES5
• HEY2