Seroatlas · Human Serome Atlas

H3-5

Histone H3.3C

Also known as: H3.3C, H3.5, H3C_HUMAN, H3F3C

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6NXT2
Gene
H3-5
Ensembl
ENSG00000188375
Chromosome
12
Canonical length
135 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Acrosome,Principal piece

OverviewNCBI Gene

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. [provided by RefSeq, Oct 2015]

Canonical amino-acid sequenceUniProt

135 residues, UniProt reviewed canonical sequence.

>Q6NXT2|H3-5
     1  MARTKQTARK STGGKAPRKQ LATKAARKST PSTCGVKPHR YRPGTVALRE IRRYQKSTEL
    61  LIRKLPFQRL VREIAQDFNT DLRFQSAAVG ALQEASEAYL VGLLEDTNLC AIHAKRVTIM
   121  PKDIQLARRI RGERA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against H3-5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.54
Highest tissue expression
20 nTPM

Expression across tissuesHPA

Tissue

  • testis: 20 nTPM
  • bone marrow: 2.9 nTPM
  • spleen: 1.3 nTPM
  • appendix: 1 nTPM
  • skin: 0.9 nTPM
  • ovary: 0.8 nTPM

Single-cell type

  • late primary spermatocytes: 231 nCPM
  • early spermatids: 100 nCPM
  • late spermatids: 15 nCPM
  • early primary spermatocytes: 13 nCPM
  • esophageal apical cells: 3.4 nCPM
  • gastric chief cells: 3.3 nCPM

Immune cell

  • neutrophil: 73 nTPM
  • eosinophil: 42 nTPM
  • total PBMC: 41 nTPM
  • basophil: 37 nTPM
  • memory B-cell: 16 nTPM
  • intermediate monocyte: 16 nTPM

Brain region

  • cerebral cortex: 0.8 nTPM
  • basal ganglia: 0.1 nTPM
  • medulla oblongata: 0.1 nTPM
  • pons: 0.1 nTPM
  • spinal cord: 0.1 nTPM
  • white matter: 0.1 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about H3-5.

Disease | ImmuneIEDB

Conditions an epitope on H3-5 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0
gnomAD pLI
0
DepMap mean gene effect
0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads H3-5 as an antibody target. Whether an autoantibody or antibody against H3-5 could matter depends on whether native H3-5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

H3-5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label H3-5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/H3-5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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