GUK1

Protein identityUniProt · HPA

UniProt accession

Q16774

Gene

GUK1

Ensembl

ENSG00000143774

Chromosome

1

Canonical length

197 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Golgi apparatus,Plasma membrane

OverviewNCBI Gene

The protein encoded by this gene is an enzyme that catalyzes the transfer of a phosphate group from ATP to guanosine monophosphate (GMP) to form guanosine diphosphate (GDP). The encoded protein is thought to be a good target for cancer chemotherapy. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Canonical amino-acid sequenceUniProt

197 residues, UniProt reviewed canonical sequence.

>Q16774|GUK1
     1  MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV
    61  MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI
   121  SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE
   181  LKEALSEEIK KAQRTGA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GUK1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

464 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about GUK1.

Disease | AllUniProt

Conditions GUK1 is implicated in, by any mechanism.

• Mitochondrial DNA depletion syndrome 21 (MTDPS21) MIM:621071

Disease | GeneticClinVar

4 pathogenic / likely-pathogenic of 74 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Mitochondrial dna depletion syndrome 21

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.43

gnomAD pLI

0

gnomAD missense Z

0.08

DepMap mean gene effect

-1.26

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• dGMP metabolic process
• GDP-mannose metabolic process
• glycoprotein transport
• nucleobase-containing small molecule interconversion
• purine nucleotide metabolic process
• xenobiotic metabolic process
• dATP metabolic process
• dGDP biosynthetic process
• GDP biosynthetic process

Molecular functions

• ATP binding
• GMP kinase activity

Cellular components

• cytosol
• mitochondrion
• photoreceptor inner segment

Protein domainsUniProt · Pfam · InterPro

• Guanylate kinase-like domain
• Guanylate kinase/L-type calcium channel beta subunit
• Guanylate kinase, conserved site
• P-loop containing nucleoside triphosphate hydrolase
• Guanylate kinase
• Guanylate kinase

KeywordsUniProt

• Acetylation
• ATP-binding
• Cytoplasm
• Kinase
• Mitochondrion
• Nucleotide-binding
• Transferase

InteractionsUniProt · HPA

Protein binding partners of GUK1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RD3