GSTO1

Protein identityUniProt · HPA

UniProt accession

P78417

Gene

GSTO1

Ensembl

ENSG00000148834

Chromosome

10

Canonical length

241 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Canonical amino-acid sequenceUniProt

241 residues, UniProt reviewed canonical sequence.

>P78417|GSTO1
     1  MSGESARSLG KGSAPPGPVP EGSIRIYSMR FCPFAERTRL VLKAKGIRHE VININLKNKP
    61  EWFFKKNPFG LVPVLENSQG QLIYESAITC EYLDEAYPGK KLLPDDPYEK ACQKMILELF
   121  SKVPSLVGSF IRSQNKEDYA GLKEEFRKEF TKLEEVLTNK KTTFFGGNSI SMIDYLIWPW
   181  FERLEAMKLN ECVDHTPKLK LWMAAMKEDP TVSALLTSEK DWQGFLELYL QNSPEACDYG
   241  L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GSTO1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

614 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for GSTO1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.75

gnomAD pLI

0

gnomAD missense Z

0.39

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to arsenic-containing substance
• glutathione metabolic process
• L-ascorbic acid metabolic process
• negative regulation of release of sequestered calcium ion into cytosol
• positive regulation of release of sequestered calcium ion into cytosol
• regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
• regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
• xenobiotic catabolic process
• positive regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion

Molecular functions

• glutathione dehydrogenase (ascorbate) activity
• glutathione transferase activity
• methylarsonate reductase activity
• oxidoreductase activity

Cellular components

• cytoplasm
• cytosol
• extracellular exosome

Protein domainsUniProt · Pfam · InterPro

• Glutathione S-transferase, N-terminal
• Glutathione S-transferase, C-terminal
• Glutathione S-transferase, omega-class
• Glutathione S-transferase, C-terminal-like
• Thioredoxin-like superfamily
• Glutathione S-transferase, C-terminal domain superfamily
• Glutathione transferase family
• Glutathione S-transferase Omega/HSP26
• Glutathione S-transferase, N-terminal domain
• Glutathione S-transferase, C-terminal domain
• Glutathione S-transferase Omega/Tau-like

KeywordsUniProt

• Acetylation
• Cytoplasm
• Oxidoreductase
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of GSTO1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HSPB1