GSTM4

Protein identityUniProt · HPA

UniProt accession

Q03013

Gene

GSTM4

Ensembl

ENSG00000168765

Chromosome

1

Canonical length

218 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Microtubules,Cytokinetic bridge,Primary cilium,Primary cilium tip,Primary cilium transition zone,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. Multiple transcript variants, each encoding a distinct protein isoform, have been identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

218 residues, UniProt reviewed canonical sequence.

>Q03013|GSTM4
     1  MSMTLGYWDI RGLAHAIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK FKLGLDFPNL
    61  PYLIDGAHKI TQSNAILCYI ARKHNLCGET EEEKIRVDIL ENQAMDVSNQ LARVCYSPDF
   121  EKLKPEYLEE LPTMMQHFSQ FLGKRPWFVG DKITFVDFLA YDVLDLHRIF EPNCLDAFPN
   181  LKDFISRFEG LEKISAYMKS SRFLPKPLYT RVAVWGNK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GSTM4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

128 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.62

gnomAD pLI

0

gnomAD missense Z

0.13

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• glutathione metabolic process
• long-chain fatty acid biosynthetic process
• nitrobenzene metabolic process
• xenobiotic catabolic process

Molecular functions

• enzyme binding
• glutathione binding
• glutathione transferase activity
• leukotriene-C4 synthase activity
• protein homodimerization activity

Cellular components

• cytoplasm
• cytosol

Protein domainsUniProt · Pfam · InterPro

• Glutathione S-transferase, Mu class
• Glutathione S-transferase, N-terminal
• Glutathione S-transferase, C-terminal
• Glutathione S-transferase, C-terminal-like
• Thioredoxin-like superfamily
• Glutathione S-transferase, C-terminal domain superfamily
• Glutathione transferase family
• Glutathione S-transferase superfamily
• Glutathione S-transferase, C-terminal domain
• Glutathione S-transferase, N-terminal domain

KeywordsUniProt

• Cytoplasm
• Lipid metabolism
• Lyase
• Transferase

InteractionsUniProt · HPA

Protein binding partners of GSTM4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GSTM3
• GSTM5