GRK4

Protein identityUniProt · HPA

UniProt accession

P32298

Gene

GRK4

Ensembl

ENSG00000125388

Chromosome

4

Canonical length

578 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol,Calyx

OverviewNCBI Gene

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Canonical amino-acid sequenceUniProt

578 residues, UniProt reviewed canonical sequence.

>P32298|GRK4
     1  MELENIVANS LLLKARQGGY GKKSGRSKKW KEILTLPPVS QCSELRHSIE KDYSSLCDKQ
    61  PIGRRLFRQF CDTKPTLKRH IEFLDAVAEY EVADDEDRSD CGLSILDRFF NDKLAAPLPE
   121  IPPDVVTECR LGLKEENPSK KAFEECTRVA HNYLRGEPFE EYQESSYFSQ FLQWKWLERQ
   181  PVTKNTFRHY RVLGKGGFGE VCACQVRATG KMYACKKLQK KRIKKRKGEA MALNEKRILE
   241  KVQSRFVVSL AYAYETKDAL CLVLTIMNGG DLKFHIYNLG NPGFDEQRAV FYAAELCCGL
   301  EDLQRERIVY RDLKPENILL DDRGHIRISD LGLATEIPEG QRVRGRVGTV GYMAPEVVNN
   361  EKYTFSPDWW GLGCLIYEMI QGHSPFKKYK EKVKWEEVDQ RIKNDTEEYS EKFSEDAKSI
   421  CRMLLTKNPS KRLGCRGEGA AGVKQHPVFK DINFRRLEAN MLEPPFCPDP HAVYCKDVLD
   481  IEQFSVVKGI YLDTADEDFY ARFATGCVSI PWQNEMIESG CFKDINKSES EEALPLDLDK
   541  NIHTPVSRPN RGFFYRLFRR GGCLTMVPSE KEVEPKQC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GRK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

40 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.09

gnomAD pLI

0

gnomAD missense Z

-0.58

DepMap mean gene effect

0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• receptor internalization
• regulation of G protein-coupled receptor signaling pathway
• regulation of opsin-mediated signaling pathway
• regulation of signal transduction
• signal transduction

Molecular functions

• ATP binding
• protein kinase activity
• rhodopsin kinase activity

Cellular components

• cell cortex
• cytoplasm
• cytosol
• photoreceptor disc membrane
• plasma membrane
• sperm glycocalyx

Protein domainsUniProt · Pfam · InterPro

• GPCR kinase
• Protein kinase domain
• AGC-kinase, C-terminal
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• RGS domain
• Protein kinase, ATP binding site
• RGS domain superfamily
• RGS, subdomain 2
• Protein kinase domain
• Regulator of G protein signaling domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cytoplasm
• Kinase
• Lipoprotein
• Nucleotide-binding
• Palmitate
• Phosphoprotein
• Serine/threonine-protein kinase
• Transferase

InteractionsUniProt · HPA

Protein binding partners of GRK4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DRD3
• GRK6