GRK3

Protein identityUniProt · HPA

UniProt accession

P35626

Gene

GRK3

Ensembl

ENSG00000100077

Chromosome

22

Canonical length

688 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Plasma membrane

OverviewNCBI Gene

The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

688 residues, UniProt reviewed canonical sequence.

>P35626|GRK3
     1  MADLEAVLAD VSYLMAMEKS KATPAARASK RIVLPEPSIR SVMQKYLAER NEITFDKIFN
    61  QKIGFLLFKD FCLNEINEAV PQVKFYEEIK EYEKLDNEED RLCRSRQIYD AYIMKELLSC
   121  SHPFSKQAVE HVQSHLSKKQ VTSTLFQPYI EEICESLRGD IFQKFMESDK FTRFCQWKNV
   181  ELNIHLTMNE FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER
   241  IMLSLVSTGD CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE
   301  IILGLEHMHN RFVVYRDLKP ANILLDEHGH ARISDLGLAC DFSKKKPHAS VGTHGYMAPE
   361  VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTVN VELPDTFSPE
   421  LKSLLEGLLQ RDVSKRLGCH GGGSQEVKEH SFFKGVDWQH VYLQKYPPPL IPPRGEVNAA
   481  DAFDIGSFDE EDTKGIKLLD CDQELYKNFP LVISERWQQE VTETVYEAVN ADTDKIEARK
   541  RAKNKQLGHE EDYALGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL
   601  LTMEQILSVE ETQIKDKKCI LFRIKGGKQF VLQCESDPEF VQWKKELNET FKEAQRLLRR
   661  APKFLNKPRS GTVELPKPSL CHRNSNGL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GRK3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

20 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about GRK3.

Disease | ImmuneIEDB

Conditions an epitope on GRK3 was assayed in.

• narcolepsy B cell
• multiple sclerosis B cell
• peripheral nervous system disease B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.36

gnomAD pLI

0.71

DepMap mean gene effect

0

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• desensitization of G protein-coupled receptor signaling pathway
• G protein-coupled receptor signaling pathway
• receptor internalization
• signal transduction

Molecular functions

• ATP binding
• beta-adrenergic receptor kinase activity
• G protein-coupled receptor binding
• G protein-coupled receptor kinase activity
• protein kinase activity

Cellular components

• cytosol
• plasma membrane
• postsynapse
• presynapse

Protein domainsUniProt · Pfam · InterPro

• GPCR kinase
• Protein kinase domain
• AGC-kinase, C-terminal
• Pleckstrin homology domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• PH-like domain superfamily
• RGS domain
• Protein kinase, ATP binding site
• RGS domain superfamily
• RGS, subdomain 2
• Protein kinase domain
• PH domain
• Regulator of G protein signaling domain

KeywordsUniProt

• ATP-binding
• Cell projection
• Kinase
• Nucleotide-binding
• Serine/threonine-protein kinase
• Synapse
• Transferase
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of GRK3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GIT1
• GRK2