GAPDHS

Protein identityUniProt · HPA

UniProt accession

O14556

Gene

GAPDHS

Ensembl

ENSG00000105679

Chromosome

19

Canonical length

408 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Centrosome,Principal piece

Quaternary structure

Homotetramer

OverviewNCBI Gene

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

408 residues, UniProt reviewed canonical sequence.

>O14556|GAPDHS
     1  MSKRDIVLTN VTVVQLLRQP CPVTRAPPPP EPKAEVEPQP QPEPTPVREE IKPPPPPLPP
    61  HPATPPPKMV SVARELTVGI NGFGRIGRLV LRACMEKGVK VVAVNDPFID PEYMVYMFKY
   121  DSTHGRYKGS VEFRNGQLVV DNHEISVYQC KEPKQIPWRA VGSPYVVEST GVYLSIQAAS
   181  DHISAGAQRV VISAPSPDAP MFVMGVNEND YNPGSMNIVS NASCTTNCLA PLAKVIHERF
   241  GIVEGLMTTV HSYTATQKTV DGPSRKAWRD GRGAHQNIIP ASTGAAKAVT KVIPELKGKL
   301  TGMAFRVPTP DVSVVDLTCR LAQPAPYSAI KEAVKAAAKG PMAGILAYTE DEVVSTDFLG
   361  DTHSSIFDAK AGIALNDNFV KLISWYDNEY GYSHRVVDLL RYMFSRDK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GAPDHS can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

261 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.81

gnomAD pLI

0

gnomAD missense Z

0.34

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• flagellated sperm motility
• glucose metabolic process
• glycolytic process
• positive regulation of glycolytic process

Molecular functions

• glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
• NAD binding
• NADP binding

Cellular components

• cytosol
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Glyceraldehyde-3-phosphate dehydrogenase, type I
• Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain
• Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain
• Glyceraldehyde 3-phosphate dehydrogenase, active site
• Glyceraldehyde/Erythrose phosphate dehydrogenase family
• NAD(P)-binding domain superfamily
• Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
• Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain

KeywordsUniProt

• Cytoplasm
• Glycolysis
• NAD
• Oxidoreductase

InteractionsUniProt · HPA

Protein binding partners of GAPDHS in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ARRB2