Seroatlas · Human Serome Atlas

FOXB1

Forkhead box protein B1

Also known as: FKH5, FOXB1_HUMAN, HFKH-5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q99853
Gene
FOXB1
Ensembl
ENSG00000171956
Chromosome
15
Canonical length
325 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm,Mitochondria

OverviewNCBI Gene

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in several processes, including mammary gland development; nervous system development; and visual learning. Predicted to be located in chromatin and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

325 residues, UniProt reviewed canonical sequence.

>Q99853|FOXB1
     1  MPRPGRNTYS DQKPPYSYIS LTAMAIQSSP EKMLPLSEIY KFIMDRFPYY RENTQRWQNS
    61  LRHNLSFNDC FIKIPRRPDQ PGKGSFWALH PSCGDMFENG SFLRRRKRFK VLKSDHLAPS
   121  KPADAAQYLQ QQAKLRLSAL AASGTHLPQM PAAAYNLGGV AQPSGFKHPF AIENIIAREY
   181  KMPGGLAFSA MQPVPAAYPL PNQLTTMGSS LGTGWPHVYG SAGMIDSATP ISMASGDYSA
   241  YGVPLKPLCH AAGQTLPAIP VPIKPTPAAV PALPALPAPI PTLLSNSPPS LSPTSSQTAT
   301  SQSSPATPSE TLTSPASALH SVAVH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FOXB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.59
Highest tissue expression
3.9 nTPM

Expression across tissuesHPA

Tissue

  • midbrain: 3.9 nTPM
  • hypothalamus: 3.4 nTPM
  • spinal cord: 1.2 nTPM
  • testis: 0.6 nTPM
  • basal ganglia: 0.2 nTPM
  • lymph node: 0.2 nTPM

Single-cell type

  • late primary spermatocytes: 36 nCPM
  • early spermatids: 18 nCPM
  • astrocytes: 10 nCPM
  • endometrial ciliated cells: 7.9 nCPM
  • late spermatids: 6.8 nCPM
  • ependymal cells: 5.8 nCPM

Immune cell

  • T-reg: 1.1 nTPM
  • memory CD4 T-cell: 0.2 nTPM
  • naive CD4 T-cell: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM

Brain region

  • hypothalamus: 47 nTPM
  • thalamus: 21 nTPM
  • medulla oblongata: 20 nTPM
  • midbrain: 18 nTPM
  • spinal cord: 17 nTPM
  • pons: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.39
gnomAD pLI
0.91
gnomAD missense Z
1.48
DepMap mean gene effect
0.05
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FOXB1 as an antibody target. Whether an autoantibody or antibody against FOXB1 could matter depends on whether native FOXB1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FOXB1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FOXB1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/FOXB1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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