FBXO21

Protein identityUniProt · HPA

UniProt accession

O94952

Gene

FBXO21

Ensembl

ENSG00000135108

Chromosome

12

Canonical length

628 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Mitochondria

OverviewNCBI Gene

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

628 residues, UniProt reviewed canonical sequence.

>O94952|FBXO21
     1  MAAAAVDSAM EVVPALAEEA APEVAGLSCL VNLPGEVLEY ILCCGSLTAA DIGRVSSTCR
    61  RLRELCQSSG KVWKEQFRVR WPSLMKHYSP TDYVNWLEEY KVRQKAGLEA RKIVASFSKR
   121  FFSEHVPCNG FSDIENLEGP EIFFEDELVC ILNMEGRKAL TWKYYAKKIL YYLRQQKILN
   181  NLKAFLQQPD DYESYLEGAV YIDQYCNPLS DISLKDIQAQ IDSIVELVCK TLRGINSRHP
   241  SLAFKAGESS MIMEIELQSQ VLDAMNYVLY DQLKFKGNRM DYYNALNLYM HQVLIRRTGI
   301  PISMSLLYLT IARQLGVPLE PVNFPSHFLL RWCQGAEGAT LDIFDYIYID AFGKGKQLTV
   361  KECEYLIGQH VTAALYGVVN VKKVLQRMVG NLLSLGKREG IDQSYQLLRD SLDLYLAMYP
   421  DQVQLLLLQA RLYFHLGIWP EKSFCLVLKV LDILQHIQTL DPGQHGAVGY LVQHTLEHIE
   481  RKKEEVGVEV KLRSDEKHRD VCYSIGLIMK HKRYGYNCVI YGWDPTCMMG HEWIRNMNVH
   541  SLPHGHHQPF YNVLVEDGSC RYAAQENLEY NVEPQEISHP DVGRYFSEFT GTHYIPNAEL
   601  EIRYPEDLEF VYETVQNIYS AKKENIDE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FBXO21 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

108 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.31

gnomAD pLI

0.98

gnomAD missense Z

2.72

DepMap mean gene effect

-0.06

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic process
• cytokine-mediated signaling pathway
• ERK1 and ERK2 cascade
• hematopoietic stem cell differentiation
• hematopoietic stem cell homeostasis
• ubiquitin-dependent protein catabolic process

Molecular functions

• DNA binding
• ubiquitin-protein transferase activity

Cellular components

• cytosol
• ubiquitin ligase complex

Protein domainsUniProt · Pfam · InterPro

• F-box domain
• Hemimethylated DNA-binding domain
• F-box-like domain superfamily
• Hemimethylated DNA-binding domain superfamily
• Hemimethylated DNA-binding protein YccV like
• F-box-like
• Protein SirB1, N-terminal
• Transglutaminase-like superfamily

KeywordsUniProt

• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of FBXO21 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CUL1
• SKP1
• EID1