Seroatlas · Human Serome Atlas

EPPIN

Eppin

Also known as: CT71, dJ461P17.2, EPPI_HUMAN, EPPIN-WFDC6, EPPIN1, EPPIN2, EPPIN3, SPINLW1, SPINLW1-WFDC6, WAP7, WFDC7

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O95925
Gene
EPPIN
Ensembl
ENSG00000101448
Chromosome
20
Canonical length
133 aa
Protein class
Predicted intracellular proteins, Predicted secreted proteins
Secretome location
Secreted in male reproductive system
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility and exhibits antimicrobial activity against E. coli, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Canonical amino-acid sequenceUniProt

133 residues, UniProt reviewed canonical sequence.

>O95925|EPPIN
     1  MGSSGLLSLL VLFVLLANVQ GPGLTDWLFP RRCPKIREEC EFQERDVCTK DRQCQDNKKC
    61  CVFSCGKKCL DLKQDVCEMP KETGPCLAYF LHWWYDKKDN TCSMFVYGGC QGNNNNFQSK
   121  ANCLNTCKNK RFP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EPPIN can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
309 nTPM

Expression across tissuesHPA

Tissue

  • epididymis: 309 nTPM
  • testis: 75 nTPM
  • bone marrow: 2.2 nTPM
  • liver: 1.5 nTPM
  • fallopian tube: 1.2 nTPM
  • retina: 0.7 nTPM

Single-cell type

  • epididymal principal cells: 66 nCPM
  • sertoli cells: 64 nCPM
  • endometrial ciliated cells: 13 nCPM
  • late spermatids: 12 nCPM
  • respiratory ciliated cells: 10 nCPM
  • fallopian tube ciliated cells: 7.1 nCPM

Immune cell

  • basophil: 0.5 nTPM
  • naive B-cell: 0.2 nTPM
  • neutrophil: 0.2 nTPM
  • classical monocyte: 0.1 nTPM
  • gdT-cell: 0.1 nTPM
  • NK-cell: 0.1 nTPM

Brain region

  • cerebral cortex: 19 nTPM
  • white matter: 18 nTPM
  • cerebellum: 17 nTPM
  • basal ganglia: 16 nTPM
  • hippocampal formation: 15 nTPM
  • amygdala: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.19
gnomAD pLI
0
gnomAD missense Z
-0.04
DepMap mean gene effect
0.09
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EPPIN in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EPPIN as an antibody target. Whether an autoantibody or antibody against EPPIN could matter depends on whether native EPPIN is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EPPIN is annotated at the cell surface, where native EPPIN is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EPPIN as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EPPIN. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...