EPHA5

Protein identityUniProt · HPA

UniProt accession

P54756

Gene

EPHA5

Ensembl

ENSG00000145242

Chromosome

4

Canonical length

1037 aa

Protein class

Enzymes, Predicted membrane proteins

Subcellular location

Golgi apparatus,Vesicles,Plasma membrane

OverviewNCBI Gene

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]

Canonical amino-acid sequenceUniProt

1037 residues, UniProt reviewed canonical sequence.

>P54756|EPHA5
     1  MRGSGPRGAG RRRPPSGGGD TPITPASLAG CYSAPRRAPL WTCLLLCAAL RTLLASPSNE
    61  VNLLDSRTVM GDLGWIAFPK NGWEEIGEVD ENYAPIHTYQ VCKVMEQNQN NWLLTSWISN
   121  EGASRIFIEL KFTLRDCNSL PGGLGTCKET FNMYYFESDD QNGRNIKENQ YIKIDTIAAD
   181  ESFTELDLGD RVMKLNTEVR DVGPLSKKGF YLAFQDVGAC IALVSVRVYY KKCPSVVRHL
   241  AVFPDTITGA DSSQLLEVSG SCVNHSVTDE PPKMHCSAEG EWLVPIGKCM CKAGYEEKNG
   301  TCQVCRPGFF KASPHIQSCG KCPPHSYTHE EASTSCVCEK DYFRRESDPP TMACTRPPSA
   361  PRNAISNVNE TSVFLEWIPP ADTGGRKDVS YYIACKKCNS HAGVCEECGG HVRYLPRQSG
   421  LKNTSVMMVD LLAHTNYTFE IEAVNGVSDL SPGARQYVSV NVTTNQAAPS PVTNVKKGKI
   481  AKNSISLSWQ EPDRPNGIIL EYEIKYFEKD QETSYTIIKS KETTITAEGL KPASVYVFQI
   541  RARTAAGYGV FSRRFEFETT PVFAASSDQS QIPVIAVSVT VGVILLAVVI GVLLSGSCCE
   601  CGCGRASSLC AVAHPSLIWR CGYSKAKQDP EEEKMHFHNG HIKLPGVRTY IDPHTYEDPN
   661  QAVHEFAKEI EASCITIERV IGAGEFGEVC SGRLKLPGKR ELPVAIKTLK VGYTEKQRRD
   721  FLGEASIMGQ FDHPNIIHLE GVVTKSKPVM IVTEYMENGS LDTFLKKNDG QFTVIQLVGM
   781  LRGISAGMKY LSDMGYVHRD LAARNILINS NLVCKVSDFG LSRVLEDDPE AAYTTRGGKI
   841  PIRWTAPEAI AFRKFTSASD VWSYGIVMWE VVSYGERPYW EMTNQDVIKA VEEGYRLPSP
   901  MDCPAALYQL MLDCWQKERN SRPKFDEIVN MLDKLIRNPS SLKTLVNASC RVSNLLAEHS
   961  PLGSGAYRSV GEWLEAIKMG RYTEIFMENG YSSMDAVAQV TLEDLRRLGV TLVGHQKKIM
  1021  NSLQEMKVQL VNGMVPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EPHA5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

11 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.52

gnomAD pLI

0

gnomAD missense Z

1.36

DepMap mean gene effect

-0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• axon guidance
• ephrin receptor signaling pathway
• hippocampus development
• neuron development
• positive regulation of CREB transcription factor activity
• regulation of actin cytoskeleton organization
• regulation of GTPase activity
• regulation of insulin secretion involved in cellular response to glucose stimulus

Molecular functions

• ATP binding
• ephrin receptor activity
• GPI-linked ephrin receptor activity
• transmembrane-ephrin receptor activity

Cellular components

• axon
• dendrite
• external side of plasma membrane
• neuronal cell body
• perinuclear region of cytoplasm
• plasma membrane
• rough endoplasmic reticulum

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Ephrin, ligand binding domain
• Serine-threonine/tyrosine-protein kinase, catalytic domain
• Tyrosine-protein kinase, receptor class V, conserved site
• Sterile alpha motif domain
• Fibronectin type III
• Tyrosine-protein kinase, active site
• Galactose-binding-like domain superfamily
• Protein kinase-like domain superfamily
• Sterile alpha motif/pointed domain superfamily
• Immunoglobulin-like fold
• Ephrin receptor type-A /type-B
• Protein kinase, ATP binding site
• Tyrosine-protein kinase, catalytic domain
• Ephrin, transmembrane domain
• Fibronectin type III superfamily
• Ephrin receptor tyrosine kinases
• Fibronectin type III domain
• SAM domain (Sterile alpha motif)
• Ephrin receptor ligand binding domain
• Protein tyrosine and serine/threonine kinase
• Ephrin type-A receptor 2 transmembrane domain
• Ephrin cysteine rich domain
• Ephrin type-A receptor 5, ligand binding domain

KeywordsUniProt

• ATP-binding
• Cell membrane
• Cell projection
• Glycoprotein
• Kinase
• Membrane
• Neurogenesis
• Nucleotide-binding
• Phosphoprotein
• Receptor
• Repeat
• Signal
• Transferase
• Transmembrane
• Transmembrane helix
• Tyrosine-protein kinase

InteractionsUniProt · HPA

Protein binding partners of EPHA5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• EPHA2