Seroatlas · Human Serome Atlas

EFNB3

Ephrin-B3

Also known as: EFNB3_HUMAN, EPLG8, LERK-8

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q15768
Gene
EFNB3
Ensembl
ENSG00000108947
Chromosome
17
Canonical length
340 aa
Protein class
Cancer-related genes, Predicted membrane proteins

OverviewNCBI Gene

EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

340 residues, UniProt reviewed canonical sequence.

>Q15768|EFNB3
     1  MGPPHSGPGG VRVGALLLLG VLGLVSGLSL EPVYWNSANK RFQAEGGYVL YPQIGDRLDL
    61  LCPRARPPGP HSSPNYEFYK LYLVGGAQGR RCEAPPAPNL LLTCDRPDLD LRFTIKFQEY
   121  SPNLWGHEFR SHHDYYIIAT SDGTREGLES LQGGVCLTRG MKVLLRVGQS PRGGAVPRKP
   181  VSEMPMERDR GAAHSLEPGK ENLPGDPTSN ATSRGAEGPL PPPSMPAVAG AAGGLALLLL
   241  GVAGAGGAMC WRRRRAKPSE SRHPGPGSFG RGGSLGLGGG GGMGPREAEP GELGIALRGG
   301  GAADPPFCPH YEKVSGDYGH PVYIVQDGPP QSPPNIYYKV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EFNB3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.51
Highest tissue expression
42 nTPM

Expression across tissuesHPA

Tissue

  • basal ganglia: 42 nTPM
  • hypothalamus: 31 nTPM
  • cerebral cortex: 28 nTPM
  • hippocampal formation: 18 nTPM
  • amygdala: 16 nTPM
  • parathyroid gland: 11 nTPM

Single-cell type

  • respiratory ciliated cells: 24 nCPM
  • ependymal cells: 22 nCPM
  • other brain neurons: 22 nCPM
  • brain inhibitory neurons: 19 nCPM
  • epididymal efferent duct ciliated cells: 17 nCPM
  • hepatic stellate cells: 13 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • hypothalamus: 82 nTPM
  • basal ganglia: 56 nTPM
  • cerebral cortex: 56 nTPM
  • hippocampal formation: 48 nTPM
  • amygdala: 41 nTPM
  • midbrain: 27 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.61
gnomAD pLI
0.32
gnomAD missense Z
1.57
DepMap mean gene effect
0
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EFNB3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EFNB3 as an antibody target. Whether an autoantibody or antibody against EFNB3 could matter depends on whether native EFNB3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EFNB3 is annotated at the cell surface, where native EFNB3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EFNB3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EFNB3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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