Seroatlas · Human Serome Atlas

EFNA5

Ephrin-A5

Also known as: AF1, EFNA5_HUMAN, EPLG7, LERK7

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P52803
Gene
EFNA5
Ensembl
ENSG00000184349
Chromosome
5
Canonical length
228 aa
Protein class
Cancer-related genes, Predicted membrane proteins, RAS pathway related proteins
Subcellular location
Vesicles,Cytosol

OverviewNCBI Gene

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

228 residues, UniProt reviewed canonical sequence.

>P52803|EFNA5
     1  MLHVEMLTLV FLVLWMCVFS QDPGSKAVAD RYAVYWNSSN PRFQRGDYHI DVCINDYLDV
    61  FCPHYEDSVP EDKTERYVLY MVNFDGYSAC DHTSKGFKRW ECNRPHSPNG PLKFSEKFQL
   121  FTPFSLGFEF RPGREYFYIS SAIPDNGRRS CLKLKVFVRP TNSCMKTIGV HDRVFDVNDK
   181  VENSLEPADD TVHESAEPSR GENAAQTPRI PSRLLAILLF LLAMLLTL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EFNA5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
12 nTPM

Expression across tissuesHPA

Tissue

  • skin: 12 nTPM
  • cervix: 9.5 nTPM
  • salivary gland: 8.6 nTPM
  • pituitary gland: 7.8 nTPM
  • esophagus: 7.2 nTPM
  • vagina: 6.8 nTPM

Single-cell type

  • pituicytes/fscs: 1,942 nCPM
  • distal convoluted tubule cells: 1,532 nCPM
  • mesothelial cells: 1,167 nCPM
  • salivary ionocytes: 1,075 nCPM
  • lactotrophs: 946 nCPM
  • renal connecting tubule cells: 850 nCPM

Immune cell

  • gdT-cell: 1 nTPM
  • memory CD8 T-cell: 0.9 nTPM
  • NK-cell: 0.7 nTPM
  • naive CD8 T-cell: 0.4 nTPM
  • memory CD4 T-cell: 0.2 nTPM
  • basophil: 0.1 nTPM

Brain region

  • cerebral cortex: 45 nTPM
  • hypothalamus: 38 nTPM
  • white matter: 27 nTPM
  • basal ganglia: 26 nTPM
  • cerebellum: 16 nTPM
  • pons: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.3
gnomAD pLI
0.96
gnomAD missense Z
1.52
DepMap mean gene effect
-0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EFNA5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EFNA5 as an antibody target. Whether an autoantibody or antibody against EFNA5 could matter depends on whether native EFNA5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EFNA5 is annotated at the cell surface, where native EFNA5 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EFNA5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EFNA5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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