Seroatlas · Human Serome Atlas

EFHB

EF-hand domain-containing family member B

Also known as: CFAP21, EFHB_HUMAN, FLJ25200

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N7U6
Gene: EFHB
Ensembl: ENSG00000163576
Chromosome: 3
Canonical length: 833 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nuclear bodies,Vesicles,Cytosol,Connecting piece

OverviewNCBI Gene

Enables calcium ion sensor activity. Involved in regulation of calcineurin-NFAT signaling cascade and regulation of store-operated calcium entry. Located in axonemal microtubule. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

833 residues, UniProt reviewed canonical sequence.

>Q8N7U6|EFHB
     1  MNMEIGHPHE GKDDLGDKRV IMGTKFPMEL GIRVGLGKED SRCGESPVVS NKCEGRMAPP
    61  ETKFPLSKGL EMGLERQNIS RTVMQRGSLG VDSVSASQGT KPSLLPGRMG LENESLLAGY
   121  THERIIQPPL GRVCGSSQAA GSRRAPLASG PEGVEELVGK PAFVMEPRQE MEKESTCVLM
   181  KPNTEIKLPV EVDIGLTQAE GPDETKNTEP QMGLVIEPPQ CQFAQQHEQR KEAGNIESGV
   241  EPPDRIRPIY SGKFFDRTPC WPSAGKVIPV GYRVATCLTE KLPRLITPPE AKKYFNFRYP
   301  PAGVERVFYG RANDPQIAPY LTHGIRSKIS VLANTLINPQ PITTFQQKIK DKKESIYLSN
   361  RRAPLGKSHD QAPGLPKGMD TTNTTFGTAV IKEYSAKDVV NPPKSYEEVF KEGNEGHDLY
   421  VVSHNDYYAG EAKNRKYNPS SFHRCSVYGV PTPHFNDGRA MAKSLYWLHE LQMKRGAKFV
   481  SKRADDFKEK FQHKLGRVLD PIAETMNVPP DCTFGACLRP EEYGVGDLIH NRLPDEYLRG
   541  KDRQRALIAA VRHHLKKVNY QKFDTLLAAF RHYDKKGDGM IDKDELQEAC DQANLSLDDK
   601  LLDQLFDYCD VDNDGFINYL EFANFLNWKD KMLLKEYEER VIIKGRKPDC VNPTEANVEE
   661  PEQTLLIKPE DIVLKEAGST EKTLRTLLRP SDKVSNYYKT TSSEINAIVG AIPSTCYPIC
   721  GVPTIRSDIP APRIRRISDR TNYGEEGSAY SLLYPTIFAR KGVFERDFFK TRSKEEIAEI
   781  LCNIGVKLSD EEFENVWNLA SKKHHRGEVC VENIRNVLDE LRHADRIKCK TLM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EFHB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 16 nTPM

Expression across tissuesHPA

Tissue

fallopian tube: 16 nTPM
testis: 15 nTPM
choroid plexus: 3.8 nTPM
cerebral cortex: 2.3 nTPM
hypothalamus: 2.3 nTPM
hippocampal formation: 2.1 nTPM

Single-cell type

epididymal efferent duct ciliated cells: 176 nCPM
respiratory ciliated cells: 152 nCPM
fallopian tube ciliated cells: 152 nCPM
ependymal cells: 136 nCPM
late primary spermatocytes: 97 nCPM
endometrial ciliated cells: 82 nCPM

Immune cell

gdT-cell: 0.3 nTPM
naive B-cell: 0.2 nTPM
classical monocyte: 0.1 nTPM
memory B-cell: 0.1 nTPM
neutrophil: 0.1 nTPM
plasmacytoid DC: 0.1 nTPM

Brain region

medulla oblongata: 15 nTPM
midbrain: 10 nTPM
spinal cord: 7.5 nTPM
choroid plexus: 5.4 nTPM
white matter: 4.6 nTPM
pons: 3.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.24
gnomAD pLI: 0
gnomAD missense Z: -0.24
DepMap mean gene effect: 0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

EF-hand domain
EF-hand domain pair
EF-Hand 1, calcium-binding site
EF-hand domain-containing protein EFHC1/EFHC2/EFHB
EF-hand domain pair
EFHB, C-terminal EF-hand domain
EFHB C-terminal EF-hand domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EFHB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EFHB as an antibody target. Whether an autoantibody or antibody against EFHB could matter depends on whether native EFHB is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EFHB is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EFHB as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...