Seroatlas · Human Serome Atlas

EEF2KMT

Protein-lysine N-methyltransferase EEF2KMT

Also known as: EF2KT_HUMAN, EFM3, FAM86A, MGC19636, SB153

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96G04
Gene: EEF2KMT
Ensembl: ENSG00000118894
Chromosome: 16
Canonical length: 330 aa
Protein class: Predicted intracellular proteins
Subcellular location: Vesicles

OverviewNCBI Gene

Enables protein-lysine N-methyltransferase activity. Involved in peptidyl-lysine trimethylation. Located in cytoplasm. Part of protein-containing complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

330 residues, UniProt reviewed canonical sequence.

>Q96G04|EEF2KMT
     1  MAPEENAGTE LLLQSFERRF LAARTLRSFP WQSLEAKLRD SSDSELLRDI LHKTVKHPVC
    61  VKHPPSVKYA RCFLSELIKK HEAVHTEPLD ELYEALAETL MAKESTQGHR SYLLPSGGSV
   121  TLSESTAIIS YGTTGLVTWD AALYLAEWAI ENPAVFTNRT VLELGSGAGL TGLAICKMCR
   181  PRAYIFSDCH SRVLEQLRGN VLLNGLSLEA DITAKLDSPR VTVAQLDWDV ATVHQLSAFQ
   241  PDVVIAADVL YCPEAIMSLV GVLRRLAACR EHQRAPEVYV AFTVRNPETC QLFTTELGRA
   301  GIRWEVEPRH EQKLFPYEEH LEMAMLNLTL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EEF2KMT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

testis: 11 nTPM
liver: 8.7 nTPM
skeletal muscle: 8.4 nTPM
parathyroid gland: 7.5 nTPM
pancreas: 7.2 nTPM
basal ganglia: 7 nTPM

Single-cell type

late spermatids: 492 nCPM
early spermatids: 204 nCPM
late primary spermatocytes: 26 nCPM
differentiating spermatogonia: 22 nCPM
adrenal medulla cells: 21 nCPM
esophageal basal cells: 21 nCPM

Immune cell

T-reg: 17 nTPM
non-classical monocyte: 14 nTPM
MAIT T-cell: 14 nTPM
intermediate monocyte: 12 nTPM
memory B-cell: 11 nTPM
naive CD8 T-cell: 11 nTPM

Brain region

thalamus: 7.4 nTPM
midbrain: 6.8 nTPM
medulla oblongata: 6.4 nTPM
choroid plexus: 6.3 nTPM
pons: 6.1 nTPM
basal ganglia: 5.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.79
gnomAD pLI: 0
DepMap mean gene effect: -0.7
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

peptidyl-lysine trimethylation

Molecular functions

protein-lysine N-methyltransferase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EEF2KMT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EEF2KMT as an antibody target. Whether an autoantibody or antibody against EEF2KMT could matter depends on whether native EEF2KMT is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EEF2KMT is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EEF2KMT as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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