E2F8

Protein identityUniProt · HPA

UniProt accession

A0AVK6

Gene

E2F8

Ensembl

ENSG00000129173

Chromosome

11

Canonical length

867 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Nucleoli,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012]

Canonical amino-acid sequenceUniProt

867 residues, UniProt reviewed canonical sequence.

>A0AVK6|E2F8
     1  MENEKENLFC EPHKRGLMKT PLKESTTANI VLAEIQPDFG PLTTPTKPKE GSQGEPWTPT
    61  ANLKMLISAV SPEIRNRDQK RGLFDNRSGL PEAKDCIHEH LSGDEFEKSQ PSRKEKSLGL
   121  LCHKFLARYP NYPNPAVNND ICLDEVAEEL NVERRRIYDI VNVLESLHMV SRLAKNRYTW
   181  HGRHNLNKTL GTLKSIGEEN KYAEQIMMIK KKEYEQEFDF IKSYSIEDHI IKSNTGPNGH
   241  PDMCFVELPG VEFRAASVNS RKDKSLRVMS QKFVMLFLVS TPQIVSLEVA AKILIGEDHV
   301  EDLDKSKFKT KIRRLYDIAN VLSSLDLIKK VHVTEERGRK PAFKWTGPEI SPNTSGSSPV
   361  IHFTPSDLEV RRSSKENCAK NLFSTRGKPN FTRHPSLIKL VKSIESDRRK INSAPSSPIK
   421  TNKAESSQNS APFPSKMAQL AAICKMQLEE QSSESRQKVK VQLARSGPCK PVAPLDPPVN
   481  AEMELTAPSL IQPLGMVPLI PSPLSSAVPL ILPQAPSGPS YAIYLQPTQA HQSVTPPQGL
   541  SPTVCTTHSS KATGSKDSTD ATTEKAANDT SKASASTRPG SLLPAPERQG AKSRTREPAG
   601  ERGSKRASML EDSGSKKKFK EDLKGLENVS ATLFPSGYLI PLTQCSSLGA ESILSGKENS
   661  SALSPNHRIY SSPIAGVIPV TSSELTAVNF PSFHVTPLKL MVSPTSVAAV PVGNSPALAS
   721  SHPVPIQNPS SAIVNFTLQH LGLISPNVQL SASPGSGIVP VSPRIESVNV APENAGTQQG
   781  RATNYDSPVP GQSQPNGQSV AVTGAQQPVP VTPKGSQLVA ESFFRTPGGP TKPTSSSCMD
   841  FEGANKTSLG TLFVPQRKLE VSTEDVH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against E2F8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.6

Highest tissue expression

13 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.36

gnomAD pLI

0.82

gnomAD missense Z

0.81

DepMap mean gene effect

0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• chorionic trophoblast cell differentiation
• fibroblast proliferation
• hepatocyte differentiation
• negative regulation of cytokinesis
• negative regulation of transcription by RNA polymerase II
• placenta development
• positive regulation of DNA endoreduplication
• positive regulation of transcription by RNA polymerase II
• regulation of transcription by RNA polymerase II
• sprouting angiogenesis
• trophoblast giant cell differentiation
• cell cycle comprising mitosis without cytokinesis

Molecular functions

• cis-regulatory region sequence-specific DNA binding
• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• DNA-binding transcription repressor activity
• DNA-binding transcription repressor activity, RNA polymerase II-specific
• identical protein binding
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• cytosol
• nucleolus
• nucleoplasm
• RNA polymerase II transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• E2F/DP family, winged-helix DNA-binding domain
• E2F Family
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• E2F/DP family winged-helix DNA-binding domain

KeywordsUniProt

• Activator
• Cell cycle
• DNA-binding
• Nucleus
• Phosphoprotein
• Repressor
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of E2F8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HIF1A