DUSP9

Protein identityUniProt · HPA

UniProt accession

Q99956

Gene

DUSP9

Ensembl

ENSG00000130829

Chromosome

X

Canonical length

384 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Endoplasmic reticulum

OverviewNCBI Gene

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product shows selectivity for members of the ERK family of MAP kinases and is localized to the cytoplasm and nucleus. Aberrant expression of this gene is associated with type 2 diabetes and cancer progression in several cell types. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Canonical amino-acid sequenceUniProt

384 residues, UniProt reviewed canonical sequence.

>Q99956|DUSP9
     1  MEGLGRSCLW LRRELSPPRP RLLLLDCRSR ELYESARIGG ALSVALPALL LRRLRRGSLS
    61  VRALLPGPPL QPPPPAPVLL YDQGGGRRRR GEAEAEAEEW EAESVLGTLL QKLREEGYLA
   121  YYLQGGFSRF QAECPHLCET SLAGRAGSSM APVPGPVPVV GLGSLCLGSD CSDAESEADR
   181  DSMSCGLDSE GATPPPVGLR ASFPVQILPN LYLGSARDSA NLESLAKLGI RYILNVTPNL
   241  PNFFEKNGDF HYKQIPISDH WSQNLSRFFP EAIEFIDEAL SQNCGVLVHC LAGVSRSVTV
   301  TVAYLMQKLH LSLNDAYDLV KRKKSNISPN FNFMGQLLDF ERSLRLEERH SQEQGSGGQA
   361  SAASNPPSFF TTPTSDGAFE LAPT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DUSP9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.39

Highest tissue expression

68 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.53

gnomAD pLI

0.82

gnomAD missense Z

1.32

DepMap mean gene effect

-0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• ERK1 and ERK2 cascade
• JNK cascade
• MAPK cascade
• negative regulation of ERK1 and ERK2 cascade
• protein dephosphorylation
• signal transduction

Molecular functions

• MAP kinase tyrosine phosphatase activity
• MAP kinase tyrosine/serine/threonine phosphatase activity
• phosphoprotein phosphatase activity
• protein serine/threonine phosphatase activity
• protein tyrosine/serine/threonine phosphatase activity
• protein tyrosine/threonine phosphatase activity

Cellular components

• cytoplasm
• cytosol
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Dual specificity phosphatase, catalytic domain
• Tyrosine-specific protein phosphatases domain
• Rhodanese-like domain
• Mitogen-activated protein (MAP) kinase phosphatase
• Dual specificity protein phosphatase domain
• Protein-tyrosine phosphatase-like
• Rhodanese-like domain superfamily
• Rhodanese-like domain
• Dual specificity phosphatase, catalytic domain

KeywordsUniProt

• Cytoplasm
• Hydrolase
• Phosphoprotein
• Protein phosphatase

InteractionsUniProt · HPA

Protein binding partners of DUSP9 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MAPK1
• MAPK14