DUOX1

Protein identityUniProt · HPA

UniProt accession

Q9NRD9

Gene

DUOX1

Ensembl

ENSG00000137857

Chromosome

15

Canonical length

1551 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes proteins encoded by this gene and the similar DUOX2 gene. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. This protein generates hydrogen peroxide and thereby plays a role in the activity of thyroid peroxidase, lactoperoxidase, and in lactoperoxidase-mediated antimicrobial defense at mucosal surfaces. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2012]

Canonical amino-acid sequenceUniProt

1551 residues, UniProt reviewed canonical sequence.

>Q9NRD9|DUOX1
     1  MGFCLALAWT LLVGAWTPLG AQNPISWEVQ RFDGWYNNLM EHRWGSKGSR LQRLVPASYA
    61  DGVYQPLGEP HLPNPRDLSN TISRGPAGLA SLRNRTVLGV FFGYHVLSDL VSVETPGCPA
   121  EFLNIRIPPG DPMFDPDQRG DVVLPFQRSR WDPETGRSPS NPRDPANQVT GWLDGSAIYG
   181  SSHSWSDALR SFSRGQLASG PDPAFPRDSQ NPLLMWAAPD PATGQNGPRG LYAFGAERGN
   241  REPFLQALGL LWFRYHNLWA QRLARQHPDW EDEELFQHAR KRVIATYQNI AVYEWLPSFL
   301  QKTLPEYTGY RPFLDPSISS EFVAASEQFL STMVPPGVYM RNASCHFQGV INRNSSVSRA
   361  LRVCNSYWSR EHPSLQSAED VDALLLGMAS QIAEREDHVL VEDVRDFWPG PLKFSRTDHL
   421  ASCLQRGRDL GLPSYTKARA ALGLSPITRW QDINPALSRS NDTVLEATAA LYNQDLSWLE
   481  LLPGGLLESH RDPGPLFSTI VLEQFVRLRD GDRYWFENTR NGLFSKKEIE EIRNTTLQDV
   541  LVAVINIDPS ALQPNVFVWH KGDPCPQPRQ LSTEGLPACA PSVVRDYFEG SGFGFGVTIG
   601  TLCCFPLVSL LSAWIVARLR MRNFKRLQGQ DRQSIVSEKL VGGMEALEWQ GHKEPCRPVL
   661  VYLQPGQIRV VDGRLTVLRT IQLQPPQKVN FVLSSNRGRR TLLLKIPKEY DLVLLFNLEE
   721  ERQALVENLR GALKESGLSI QEWELREQEL MRAAVTREQR RHLLETFFRH LFSQVLDINQ
   781  ADAGTLPLDS SQKVREALTC ELSRAEFAES LGLKPQDMFV ESMFSLADKD GNGYLSFREF
   841  LDILVVFMKG SPEEKSRLMF RMYDFDGNGL ISKDEFIRML RSFIEISNNC LSKAQLAEVV
   901  ESMFRESGFQ DKEELTWEDF HFMLRDHNSE LRFTQLCVKG VEVPEVIKDL CRRASYISQD
   961  MICPSPRVSA RCSRSDIETE LTPQRLQCPM DTDPPQEIRR RFGKKVTSFQ PLLFTEAHRE
  1021  KFQRSCLHQT VQQFKRFIEN YRRHIGCVAV FYAIAGGLFL ERAYYYAFAA HHTGITDTTR
  1081  VGIILSRGTA ASISFMFSYI LLTMCRNLIT FLRETFLNRY VPFDAAVDFH RLIASTAIVL
  1141  TVLHSVGHVV NVYLFSISPL SVLSCLFPGL FHDDGSELPQ KYYWWFFQTV PGLTGVVLLL
  1201  ILAIMYVFAS HHFRRRSFRG FWLTHHLYIL LYVLLIIHGS FALIQLPRFH IFFLVPAIIY
  1261  GGDKLVSLSR KKVEISVVKA ELLPSGVTHL RFQRPQGFEY KSGQWVRIAC LALGTTEYHP
  1321  FTLTSAPHED TLSLHIRAAG PWTTRLREIY SAPTGDRCAR YPKLYLDGPF GEGHQEWHKF
  1381  EVSVLVGGGI GVTPFASILK DLVFKSSVSC QVFCKKIYFI WVTRTQRQFE WLADIIREVE
  1441  ENDHQDLVSV HIYITQLAEK FDLRTTMLYI CERHFQKVLN RSLFTGLRSI THFGRPPFEP
  1501  FFNSLQEVHP QVRKIGVFSC GPPGMTKNVE KACQLINRQD RTHFSHHYEN F

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DUOX1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

7

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

99 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.94

gnomAD pLI

0

gnomAD missense Z

0.89

DepMap mean gene effect

-0.03

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cuticle development
• cytokine-mediated signaling pathway
• defense response
• hormone biosynthetic process
• hydrogen peroxide biosynthetic process
• hydrogen peroxide catabolic process
• positive regulation of cell motility
• positive regulation of wound healing
• response to cAMP
• response to oxidative stress
• superoxide anion generation
• thyroid hormone generation

Molecular functions

• calcium ion binding
• FAD binding
• heme binding
• NAD(P)H oxidase H2O2-forming activity
• NADP binding
• NADPH binding
• peroxidase activity
• protein heterodimerization activity
• superoxide-generating NAD(P)H oxidase activity

Cellular components

• apical plasma membrane
• cell leading edge
• cell surface
• endoplasmic reticulum
• NADPH oxidase complex
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• EF-hand domain
• Haem peroxidase superfamily
• EF-hand domain pair
• FAD-binding 8
• Ferric reductase, NAD binding domain
• Ferric reductase transmembrane component-like domain
• FAD-binding domain, ferredoxin reductase-type
• Riboflavin synthase-like beta-barrel
• EF-Hand 1, calcium-binding site
• Haem peroxidase, animal-type
• Dual oxidase, peroxidase domain
• Haem peroxidase domain superfamily, animal type
• Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
• Respiratory burst oxidase/Ferric reductase
• EF hand domain
• Ferric reductase like transmembrane component
• Animal haem peroxidase
• FAD-binding domain
• Ferric reductase NAD binding domain
• EF-hand domain pair

KeywordsUniProt

• Calcium
• Cell membrane
• Disulfide bond
• FAD
• Flavoprotein
• Glycoprotein
• Heme
• Hydrogen peroxide
• Iron
• Membrane
• Metal-binding
• NADP
• Oxidoreductase
• Peroxidase
• Repeat
• Signal
• Thyroid hormones biosynthesis
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of DUOX1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CYBA
• TPO
• TXNDC11