DGKD

Protein identityUniProt · HPA

UniProt accession

Q16760

Gene

DGKD

Ensembl

ENSG00000077044

Chromosome

2

Canonical length

1214 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homooligomer

OverviewNCBI Gene

This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1214 residues, UniProt reviewed canonical sequence.

>Q16760|DGKD
     1  MAAAAGAPPP GPPQPPPPPP PEESSDSEPE AEPGSPQKLI RKVSTSGQIR QKTIIKEGML
    61  TKQNNSFQRS KRRYFKLRGR TLYYAKTAKS IIFDEVDLTD ASVAESSTKN VNNSFTVITP
   121  CRKLILCADN RKEMEDWIAA LKTVQNREHF EPTQYSMDHF SGMHNWYACS HARPTYCNVC
   181  REALSGVTSH GLSCEVCKFK AHKRCAVRAT NNCKWTTLAS IGKDIIEDAD GIAMPHQWLE
   241  GNLPVSAKCT VCDKTCGSVL RLQDWRCLWC KAMVHTSCKE SLLTKCPLGL CKVSVIPPTA
   301  LNSIDSDGFW KASCPPSCTS PLLVFVNSKS GDNQGVKFLR RFKQLLNPAQ VFDLMNGGPH
   361  LGLRLFQKFD TFRILVCGGD GSVGWVLSEI DSLNLHKQCQ LGVLPLGTGN DLARVLGWGS
   421  ACDDDTQLPQ ILEKLERAST KMLDRWSVMA YEAKLPRQAS SSTVTEDFSE DSEVQQILFY
   481  EDSVAAHLSK ILTSDQHSVV ISSAKVLCET VKDFVARVGK AYEKTTESSE ESEVMAKKCS
   541  VLKEKLDSLL KTLDDESQAS SSLPNPPPTI AEEAEDGDGS GSICGSTGDR LVASACPARP
   601  QIFRPREQLM LRANSLKKAI RQIIEHTEKA VDEQNAQTQE QEGFVLGLSE SEEKMDHRVC
   661  PPLSHSESFG VPKGRSQRKV SKSPCEKLIS KGSLSLGSSA SLPPQPGSRD GLPALNTKIL
   721  YPNVRAGMSG SLPGGSVISR LLINADPFNS EPETLEYYTE KCVMNNYFGI GLDAKISLDF
   781  NNKRDEHPEK CRSRTKNMMW YGVLGTKELL HRTYKNLEQK VLLECDGRPI PLPSLQGIAV
   841  LNIPSYAGGT NFWGGTKEDD TFAAPSFDDK ILEVVAVFGS MQMAVSRVIR LQHHRIAQCR
   901  TVKISILGDE GVPVQVDGEA WVQPPGYIRI VHKNRAQTLT RDRAFESTLK SWEDKQKCEL
   961  PRPPSCSLHP EMLSEEEATQ MDQFGQAAGV LIHSIREIAQ SHRDMEQELA HAVNASSKSM
  1021  DRVYGKPRTT EGLNCSFVLE MVNNFRALRS ETELLLSGKM ALQLDPPQKE QLGSALAEMD
  1081  RQLRRLADTP WLCQSAEPGD EESVMLDLAK RSRSGKFRLV TKFKKEKNNK NKEAHSSLGA
  1141  PVHLWGTEEV AAWLEHLSLC EYKDIFTRHD IRGSELLHLE RRDLKDLGVT KVGHMKRILC
  1201  GIKELSRSAP AVEA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DGKD can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.37

Highest tissue expression

30 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.37

gnomAD pLI

0.07

gnomAD missense Z

2.96

DepMap mean gene effect

-0.06

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• diacylglycerol catabolic process
• diacylglycerol metabolic process
• endocytosis
• intracellular signal transduction
• lipid phosphorylation
• negative regulation of phospholipase C/protein kinase C signal transduction
• phosphatidic acid biosynthetic process
• phospholipase C-activating G protein-coupled receptor signaling pathway
• platelet activation
• positive regulation of clathrin-dependent endocytosis
• positive regulation of epidermal growth factor receptor signaling pathway
• protein transport
• signal transduction

Molecular functions

• ATP binding
• ATP-dependent diacylglycerol kinase activity
• diacylglycerol binding
• identical protein binding
• kinase binding
• protein heterodimerization activity
• protein homodimerization activity
• zinc ion binding

Cellular components

• clathrin-coated pit
• cytoplasm
• cytoplasmic vesicle
• cytosol
• nucleus
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Diacylglycerol kinase, accessory domain
• Diacylglycerol kinase, catalytic domain
• Sterile alpha motif domain
• Pleckstrin homology domain
• Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
• PH-like domain superfamily
• Sterile alpha motif/pointed domain superfamily
• NAD kinase/diacylglycerol kinase-like domain superfamily
• Inorganic polyphosphate/ATP-NAD kinase, N-terminal
• Diacylglycerol kinase
• C1-like domain superfamily
• Diacylglycerol kinase eta/delta/kappa, helical domain
• Phorbol esters/diacylglycerol binding domain (C1 domain)
• PH domain
• Diacylglycerol kinase accessory domain
• Diacylglycerol kinase catalytic domain
• SAM domain (Sterile alpha motif)
• Diacylglycerol kinase delta, helical domain
• Diacylglycerol kinase delta, SAM domain
• Diacylglycerol kinase delta, second protein kinase C conserved region 1
• Diacylglycerol kinase delta, first protein kinase C conserved region 1

KeywordsUniProt

• ATP-binding
• Cell membrane
• Coated pit
• Cytoplasm
• Endocytosis
• Kinase
• Lipid metabolism
• Membrane
• Metal-binding
• Nucleotide-binding
• Phosphoprotein
• Protein transport
• Repeat
• Transferase
• Transport
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of DGKD in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AP2A2
• DYSF