DDX60L

Protein identityUniProt · HPA

UniProt accession

Q5H9U9

Gene

DDX60L

Ensembl

ENSG00000181381

Chromosome

4

Canonical length

1706 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a member of the DExD/H-box helicase family of proteins, a subset of the super family 2 helicases. Members of the DExD/H-box helicase family share a conserved functional core comprised of two RecA-like globular domains. These domains contain conserved motifs that mediate ATP binding, ATP hydrolysis, nucleic acid binding, and RNA unwinding. In addition to functions in RNA metabolism, members of this family are involved in anti-viral immunity and act as cytosolic sensors of viral nucleic acids. The protein encoded by this gene has been shown to inhibit hepatitis C virus replication in response to interferon stimulation in cell culture. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

Canonical amino-acid sequenceUniProt

1706 residues, UniProt reviewed canonical sequence.

>Q5H9U9|DDX60L
     1  MGSKDHAVFF REMTQLILNE MPKAGYSSIL NDFVESNFFV IDGDSLLVTC LGVKSFKWGQ
    61  NLHFFYLVEC YLVDLLSNGG QFTIVFFKDA EYAYFDFPEL LSLRTALILH LQHNTNIDVQ
   121  TEFSGCLSQD WKLFLEQHYP YFLIVSEEGL SDLQTYLFNF LIIHSWGMKV NVVLSSGHES
   181  DTLRFYAYTM ESTDRNQTFS KENETVIQSA YKSLIQHLEE IRVLVLATHF EHLKWNDMME
   241  EAYQTLFLLQ HLWSEGSDIQ RVLCVTSCSL SLRMYHRVLV HSNCLSLQEV EDFCRLRCLC
   301  VAFQLHLPLS QRACSRVITC SWIRNSDSFL KMNKWCEYFI LSNLNVFGCW NLNLNHVSDL
   361  YDEQLLKNIA FYYEFESTQE PHLNLGDSIR RDYEDLWNVV SHLVKEFNVG KSFPLRTTRR
   421  HFLRQEKSVI QEISLEKMPS VGFIPMTSAV IDEFVGDMMK DLPILKSDDP VVPSLFKQKT
   481  SDELLHWHAQ RLLSDDYDRI KCHVDEQSRD PHVLDFLKKI QDYQQFYGKS LESISTKVIV
   541  TQTTRPKEDS SGASGEILQN TKPHQITKKS KKKSFLKEDQ NKAQQNDDLL FSIEEEMKNN
   601  LHSGIRKLED YLTSCASNSV KFGVEMLGLI ACFKAWKKHC RGEGKISKDL SIAVQMMKRI
   661  HSLLERYPEI LEAEHHQYIA KCLKYLGFND LANSLDPTLI GDDKNKKKYS IDIGPARFQL
   721  QYMGHYLIRD ERKDRDPRVQ DFIPNAWQQE LLDVVDKNES AVIVAPTSSG KTYASYYCME
   781  KVLRESDVGV VVYVAPAKSL VGQVAATVEN RFTKTLPAGR TLCGAFTRDY CHNVLNCQVL
   841  ITVPECFEIL LLAPHRQKWV ERIRYVIFDE VHYLGREVGA KFWELLLVII RCPFLVLSAT
   901  INNPNLLTKW LQSVKQYWKQ ADKIMEEKCI SEKQADKCLN FLQDHSYKNQ SYEVRLVLCG
   961  ERYNDLEKHI CSVKHDDVYF DHFHPCAALT TDIIEKYGFP PDLTLTPQES IQLYDTMAQV
  1021  WETWPRAQEL CPEEFILFKN KIVIKKLDAR KYEENLKAEL TNWIKNGQVK KVKRVLKNLS
  1081  PDSLSSSKDM VKMFPLLVEK LRQMDKLPAI FFLFKNDDVG KRAGSVCTFL EKTETKSHPH
  1141  TECHSYVFAI DEVLEKVRKT QKRITKKNPK KAEKLERKKV YRAEYINFLE NLKILEISED
  1201  CTYADVKALH TEITRNKDST LERVLPRVRF TRHGKELKAL AQRGIGYHHS SMYFKEKEFV
  1261  EILFVKGLIR VVTATETLAL GIHMPCKSVV FAQDSVYLDA LNYRQMSGRA GRRGQDLLGN
  1321  VYFFDIPLPK IKRLLASSVP ELRGQFPLSI TLVLRLMLLA SKGDDPEDAK AKVLSVLKHS
  1381  LLSFKRRRAM ETLKLYFLFS LQLLIKEDYL NKKGNPKKFA GLASYLHGHE PSNLVFVNFL
  1441  KRGLFHNLCK PAWKGSQQFS QDVMEKLVLV LANLFGRKYI PAKFQNANLS FSQSKVILAE
  1501  LPEDFKAALY EYNLAVMKDF ASFLLIASKS VNMKKEHQLP LSRIKFTGKE CEDSQLVSHL
  1561  MSCKKGRVAI SPFVCLSGNT DNDLLRPETI NQVILRTVGV SGTQAPLLWP WKLDNRGRRM
  1621  PLNAYVLNFY KHNCLTRLDQ KNGMRMGQLL KCLKDFAFNI QAISDSLSEL CENKRDNVVL
  1681  AFKQLSQTFY EKLQEMQIQM SQNHLE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX60L can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

48 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1

gnomAD pLI

0

gnomAD missense Z

0.11

DepMap mean gene effect

0.15

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• defense response to virus

Molecular functions

• ATP binding
• ATP hydrolysis activity
• double-stranded RNA binding
• RNA helicase activity
• single-stranded RNA binding

Cellular components

• cytoplasm

Protein domainsUniProt · Pfam · InterPro

• Helicase, C-terminal domain-like
• DEAD/DEAH-box helicase domain
• Helicase superfamily 1/2, ATP-binding domain
• P-loop containing nucleoside triphosphate hydrolase
• SKI2 subfamily ATP-dependent RNA helicases
• ATP-dependent RNA helicase DDX60, PIN-like domain
• DDX60-like, winged helix domain
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain
• ATP-dependent RNA helicase DDX60, PIN-like domain
• DDX60-like, winged helix domain
• DDX60 TPR domain

KeywordsUniProt

• ATP-binding
• Helicase
• Hydrolase
• Nucleotide-binding
• RNA-binding