Seroatlas · Human Serome Atlas

DDX25

ATP-dependent RNA helicase DDX25

Also known as: DDX25_HUMAN, GRTH

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UHL0
Gene: DDX25
Ensembl: ENSG00000109832
Chromosome: 11
Canonical length: 483 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

483 residues, UniProt reviewed canonical sequence.

>Q9UHL0|DDX25
     1  MASLLWGGDA GAAESERLNS HFSNLSQPRK NLWGIKSTAV RNIDGSINNI NEDDEEDVVD
    61  LAANSLLNKL IHQSLVESSH RVEVLQKDPS SPLYSVKTFE ELRLKEELLK GIYAMGFNRP
   121  SKIQEMALPM MLAHPPQNLI AQSQSGTGKT AAFVLAMLSR VNALELFPQC LCLAPTYELA
   181  LQTGRVVEQM GKFCVDVQVM YAIRGNRIPR GTDITKQIII GTPGTVLDWC FKLKLIDLTK
   241  IRVFVLDEAD VMIDTQGFSD HSIRIQRALP SECQMLLFSA TFEDSVWHFA ERIIPDPNVI
   301  KLRKEELTLN NIRQYYVLCE HRKDKYQALC NIYGSITIGQ AIIFCQTRRN AKWLTVEMIQ
   361  DGHQVSLLSG ELTVEQRASI IQRFRDGKEK VLITTNVCAR GIDVKQVTIV VNFDLPVKQG
   421  EEPDYETYLH RIGRTGRFGK KGLAFNMIEV DELPSLMKIQ DHFNSSIKQL NAEDMDEIEK
   481  IDY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 85 nTPM

Expression across tissuesHPA

Tissue

testis: 85 nTPM
basal ganglia: 22 nTPM
cerebral cortex: 21 nTPM
retina: 20 nTPM
hypothalamus: 19 nTPM
cerebellum: 15 nTPM

Single-cell type

late primary spermatocytes: 627 nCPM
epicardial cells: 353 nCPM
early spermatids: 297 nCPM
early primary spermatocytes: 86 nCPM
late spermatids: 71 nCPM
thyrotrophs: 37 nCPM

Immune cell

basophil: 0.5 nTPM
neutrophil: 0.3 nTPM
NK-cell: 0.2 nTPM
eosinophil: 0.1 nTPM
memory CD4 T-cell: 0.1 nTPM
naive B-cell: 0.1 nTPM

Brain region

hypothalamus: 33 nTPM
cerebral cortex: 32 nTPM
basal ganglia: 29 nTPM
white matter: 26 nTPM
cerebellum: 24 nTPM
hippocampal formation: 21 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DDX25.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 106 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Azoospermia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.31
gnomAD pLI: 0
gnomAD missense Z: 1.21
DepMap mean gene effect: 0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DDX25 as an antibody target. Whether an autoantibody or antibody against DDX25 could matter depends on whether native DDX25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DDX25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DDX25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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