Seroatlas · Human Serome Atlas

DCAF4

DDB1- and CUL4-associated factor 4

Also known as: DCAF4_HUMAN, DKFZp434K114, WDR21, WDR21A

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WV16
Gene: DCAF4
Ensembl: ENSG00000119599
Chromosome: 14
Canonical length: 495 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2009]

Canonical amino-acid sequenceUniProt

495 residues, UniProt reviewed canonical sequence.

>Q8WV16|DCAF4
     1  MNKSRWQSRR RHGRRSHQQN PWFRLRDSED RSDSRAAQPA HDSGHGDDES PSTSSGTAGT
    61  SSVPELPGFY FDPEKKRYFR LLPGHNNCNP LTKESIRQKE MESKRLRLLQ EEDRRKKIAR
   121  MGFNASSMLR KSQLGFLNVT NYCHLAHELR LSCMERKKVQ IRSMDPSALA SDRFNLILAD
   181  TNSDRLFTVN DVKVGGSKYG IINLQSLKTP TLKVFMHENL YFTNRKVNSV CWASLNHLDS
   241  HILLCLMGLA ETPGCATLLP ASLFVNSHPG IDRPGMLCSF RIPGAWSCAW SLNIQANNCF
   301  STGLSRRVLL TNVVTGHRQS FGTNSDVLAQ QFALMAPLLF NGCRSGEIFA IDLRCGNQGK
   361  GWKATRLFHD SAVTSVRILQ DEQYLMASDM AGKIKLWDLR TTKCVRQYEG HVNEYAYLPL
   421  HVHEEEGILV AVGQDCYTRI WSLHDARLLR TIPSPYPASK ADIPSVAFSS RLGGSRGAPG
   481  LLMAVGQDLY CYSYS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DCAF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 12 nTPM
skin: 12 nTPM
pancreas: 12 nTPM
thyroid gland: 11 nTPM
kidney: 9 nTPM
ovary: 8.7 nTPM

Single-cell type

differentiating spermatogonia: 41 nCPM
cytotrophoblasts: 29 nCPM
migrating cytotrophoblasts: 28 nCPM
epicardial cells: 27 nCPM
extravillous trophoblasts: 23 nCPM
endometrial luminal cells: 22 nCPM

Immune cell

memory B-cell: 12 nTPM
NK-cell: 9.1 nTPM
naive B-cell: 8.7 nTPM
T-reg: 7.2 nTPM
MAIT T-cell: 6.1 nTPM
naive CD8 T-cell: 6.1 nTPM

Brain region

pons: 13 nTPM
hypothalamus: 11 nTPM
cerebral cortex: 10 nTPM
medulla oblongata: 10 nTPM
white matter: 10 nTPM
midbrain: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.74
gnomAD pLI: 0
gnomAD missense Z: 0.42
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein ubiquitination

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DCAF4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DCAF4 as an antibody target. Whether an autoantibody or antibody against DCAF4 could matter depends on whether native DCAF4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DCAF4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DCAF4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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