Seroatlas · Human Serome Atlas

DCAF16

DDB1- and CUL4-associated factor 16

Also known as: C4orf30, DCA16_HUMAN, FLJ20280

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NXF7
Gene: DCAF16
Ensembl: ENSG00000163257
Chromosome: 4
Canonical length: 216 aa
Protein class: Predicted intracellular proteins
Subcellular location: Plasma membrane,Cytosol

OverviewNCBI Gene

Predicted to be involved in protein ubiquitination. Part of Cul4-RING E3 ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

216 residues, UniProt reviewed canonical sequence.

>Q9NXF7|DCAF16
     1  MGPRNPSPDH LSESESEEEE NISYLNESSG EEWDSSEEED SMVPNLSPLE SLAWQVKCLL
    61  KYSTTWKPLN PNSWLYHAKL LDPSTPVHIL REIGLRLSHC SHCVPKLEPI PEWPPLASCG
   121  VPPFQKPLTS PSRLSRDHAT LNGALQFATK QLSRTLSRAT PIPEYLKQIP NSCVSGCCCG
   181  WLTKTVKETT RTEPINTTYS YTDFQKAVNK LLTASL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DCAF16 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.54
Highest tissue expression: 19 nTPM

Expression across tissuesHPA

Tissue

ovary: 19 nTPM
bone marrow: 17 nTPM
skin: 14 nTPM
endometrium: 13 nTPM
pituitary gland: 13 nTPM
retina: 12 nTPM

Single-cell type

adrenal medulla cells: 75 nCPM
corticotrophs: 64 nCPM
renal collecting duct intercalated cells: 55 nCPM
sertoli cells: 54 nCPM
somatotrophs: 54 nCPM
podocytes: 52 nCPM

Immune cell

basophil: 7.7 nTPM
naive CD4 T-cell: 3.4 nTPM
MAIT T-cell: 3.3 nTPM
naive CD8 T-cell: 3.2 nTPM
naive B-cell: 2.9 nTPM
memory CD8 T-cell: 2.8 nTPM

Brain region

white matter: 30 nTPM
hypothalamus: 27 nTPM
basal ganglia: 25 nTPM
cerebellum: 24 nTPM
medulla oblongata: 24 nTPM
midbrain: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.48
gnomAD pLI: 0
gnomAD missense Z: 0.04
DepMap mean gene effect: 0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein ubiquitination

Cellular components

Protein domainsUniProt · Pfam · InterPro

DDB1- and CUL4-associated factor 16
DDB1- and CUL4-associated factor 16

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DCAF16 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DCAF16 as an antibody target. Whether an autoantibody or antibody against DCAF16 could matter depends on whether native DCAF16 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DCAF16 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DCAF16 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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