Seroatlas · Human Serome Atlas

DARS1

Aspartate--tRNA ligase, cytoplasmic

Also known as: DARS, SYDC_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P14868
Gene
DARS1
Ensembl
ENSG00000115866
Chromosome
2
Canonical length
501 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location
Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Canonical amino-acid sequenceUniProt

501 residues, UniProt reviewed canonical sequence.

>P14868|DARS1
     1  MPSASASRKS QEKPREIMDA AEDYAKERYG ISSMIQSQEK PDRVLVRVRD LTIQKADEVV
    61  WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ MVKFAANINK ESIVDVEGVV
   121  RKVNQKIGSC TQQDVELHVQ KIYVISLAEP RLPLQLDDAV RPEAEGEEEG RATVNQDTRL
   181  DNRVIDLRTS TSQAVFRLQS GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF
   241  KNNAYLAQSP QLYKQMCICA DFEKVFSIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH
   301  EVMEEIADTM VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE ALAMLREAGV
   361  EMGDEDDLST PNEKLLGHLV KEKYDTDFYI LDKYPLAVRP FYTMPDPRNP KQSNSYDMFM
   421  RGEEILSGAQ RIHDPQLLTE RALHHGIDLE KIKAYIDSFR FGAPPHAGGG IGLERVTMLF
   481  LGLHNVRQTS MFPRDPKRLT P

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DARS1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
81 nTPM

Expression across tissuesHPA

Tissue

  • retina: 81 nTPM
  • skeletal muscle: 78 nTPM
  • thymus: 59 nTPM
  • tongue: 56 nTPM
  • tonsil: 52 nTPM
  • ovary: 48 nTPM

Single-cell type

  • esophageal apical cells: 250 nCPM
  • pancreatic acinar cells: 250 nCPM
  • extravillous trophoblasts: 236 nCPM
  • migrating cytotrophoblasts: 211 nCPM
  • syncytiotrophoblasts: 204 nCPM
  • esophageal suprabasal cells: 186 nCPM

Immune cell

  • memory B-cell: 221 nTPM
  • naive B-cell: 200 nTPM
  • naive CD4 T-cell: 176 nTPM
  • MAIT T-cell: 175 nTPM
  • memory CD4 T-cell: 173 nTPM
  • NK-cell: 172 nTPM

Brain region

  • hypothalamus: 46 nTPM
  • medulla oblongata: 46 nTPM
  • white matter: 46 nTPM
  • spinal cord: 43 nTPM
  • cerebellum: 42 nTPM
  • pons: 38 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DARS1.

Disease | AllUniProt

Conditions DARS1 is implicated in, by any mechanism.

  • Hypomyelination with brainstem and spinal cord involvement and leg spasticity (HBSL) MIM:615281

Disease | GeneticClinVar

11 pathogenic / likely-pathogenic of 263 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.68
gnomAD pLI
0
DepMap mean gene effect
-1.91
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DARS1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DARS1 as an antibody target. Whether an autoantibody or antibody against DARS1 could matter depends on whether native DARS1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DARS1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DARS1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DARS1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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