CYTH4

Protein identityUniProt · HPA

UniProt accession

Q9UIA0

Gene

CYTH4

Ensembl

ENSG00000100055

Chromosome

22

Canonical length

394 aa

Protein class

Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytokinetic bridge,Centriolar satellite,Cytosol

OverviewNCBI Gene

This gene encodes a member of the PSCD family of proteins, which have an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family function as GEPs for ADP-ribosylation factors (ARFs), which are guanine nucleotide-binding proteins involved in vesicular trafficking pathways. This protein exhibits GEP activity in vitro with ARF1 and ARF5, but is inactive with ARF6. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]

Canonical amino-acid sequenceUniProt

394 residues, UniProt reviewed canonical sequence.

>Q9UIA0|CYTH4
     1  MDLCHPEPAE LSSGETEELQ RIKWHRKQLL EDIQKLKDEI ADVFAQIDCF ESAEESRMAQ
    61  KEKELCIGRK KFNMDPAKGI QYFIEHKLLT PDVQDIARFL YKGEGLNKTA IGTYLGERDP
   121  INLQVLQAFV DCHEFANLNL VQALRQFLWS FRLPGEAQKI DRMMEAFATR YCLCNPGVFQ
   181  STDTCYVLSF SIIMLNTSLH NPNVRDRPPF ERFVSMNRGI NNGSDLPEDQ LRNLFDSIKS
   241  EPFSIPEDDG NDLTHTFFNP DREGWLLKLG GRVKTWKRRW FILTDNCLYY FEFTTDKEPR
   301  GIIPLENLSV QKVDDPKKPF CLELYNPSCR GQKIKACKTD GDGRVVEGKH ESYRISATSA
   361  EERDQWIESI RASITRVPFY DLVSTRKKKI ASKQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CYTH4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

64 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.64

gnomAD pLI

0

gnomAD missense Z

1.43

DepMap mean gene effect

0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of ARF protein signal transduction

Molecular functions

• guanyl-nucleotide exchange factor activity
• lipid binding

Cellular components

• centriolar satellite
• cytosol
• Golgi membrane
• intercellular bridge
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Sec7 domain
• Pleckstrin homology domain
• PH-like domain superfamily
• Sec7, C-terminal domain superfamily
• Sec7 domain superfamily
• PH domain
• Sec7 domain

KeywordsUniProt

• Cell membrane
• Coiled coil
• Guanine-nucleotide releasing factor
• Lipid-binding
• Membrane

InteractionsUniProt · HPA

Protein binding partners of CYTH4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CCDC120
• IPCEF1