Seroatlas · Human Serome Atlas

COL8A1

Collagen alpha-1(VIII) chain

Also known as: C3orf7, CO8A1_HUMAN, MGC9568

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P27658
Gene: COL8A1
Ensembl: ENSG00000144810
Chromosome: 3
Canonical length: 744 aa
Protein class: Plasma proteins, Predicted secreted proteins
Subcellular location: Vesicles
Secretome location: Secreted to extracellular matrix
Quaternary structure: Homotrimer

OverviewNCBI Gene

This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]

Canonical amino-acid sequenceUniProt

744 residues, UniProt reviewed canonical sequence.

>P27658|COL8A1
     1  MAVLPGPLQL LGVLLTISLS SIRLIQAGAY YGIKPLPPQI PPQMPPQIPQ YQPLGQQVPH
    61  MPLAKDGLAM GKEMPHLQYG KEYPHLPQYM KEIQPAPRMG KEAVPKKGKE IPLASLRGEQ
   121  GPRGEPGPRG PPGPPGLPGH GIPGIKGKPG PQGYPGVGKP GMPGMPGKPG AMGMPGAKGE
   181  IGQKGEIGPM GIPGPQGPPG PHGLPGIGKP GGPGLPGQPG PKGDRGPKGL PGPQGLRGPK
   241  GDKGFGMPGA PGVKGPPGMH GPPGPVGLPG VGKPGVTGFP GPQGPLGKPG APGEPGPQGP
   301  IGVPGVQGPP GIPGIGKPGQ DGIPGQPGFP GGKGEQGLPG LPGPPGLPGI GKPGFPGPKG
   361  DRGMGGVPGA LGPRGEKGPI GAPGIGGPPG EPGLPGIPGP MGPPGAIGFP GPKGEGGIVG
   421  PQGPPGPKGE PGLQGFPGKP GFLGEVGPPG MRGLPGPIGP KGEAGQKGVP GLPGVPGLLG
   481  PKGEPGIPGD QGLQGPPGIP GIGGPSGPIG PPGIPGPKGE PGLPGPPGFP GIGKPGVAGL
   541  HGPPGKPGAL GPQGQPGLPG PPGPPGPPGP PAVMPPTPPP QGEYLPDMGL GIDGVKPPHA
   601  YGAKKGKNGG PAYEMPAFTA ELTAPFPPVG APVKFNKLLY NGRQNYNPQT GIFTCEVPGV
   661  YYFAYHVHCK GGNVWVALFK NNEPVMYTYD EYKKGFLDQA SGSAVLLLRP GDRVFLQMPS
   721  EQAAGLYAGQ YVHSSFSGYL LYPM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against COL8A1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.66
Highest tissue expression: 226 nTPM

Expression across tissuesHPA

Tissue

blood vessel: 226 nTPM
heart muscle: 35 nTPM
lung: 31 nTPM
smooth muscle: 29 nTPM
adipose tissue: 28 nTPM
thyroid gland: 26 nTPM

Single-cell type

mesothelial cells: 974 nCPM
alveolar cells type 1: 972 nCPM
pituicytes/fscs: 892 nCPM
retinal pigment epithelial cells: 874 nCPM
ependymal cells: 458 nCPM
epicardial cells: 423 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

choroid plexus: 8.6 nTPM
midbrain: 8.3 nTPM
pons: 6.7 nTPM
hypothalamus: 6.4 nTPM
medulla oblongata: 6.1 nTPM
basal ganglia: 5.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.5
gnomAD pLI: 0.41
gnomAD missense Z: 1.44
DepMap mean gene effect: -0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

extracellular matrix structural constituent conferring tensile strength

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of COL8A1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

VAC14

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads COL8A1 as an antibody target. Whether an autoantibody or antibody against COL8A1 could matter depends on whether native COL8A1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

COL8A1 is annotated as secreted, so native COL8A1 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label COL8A1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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