Seroatlas · Human Serome Atlas

CHST4

Carbohydrate sulfotransferase 4

Also known as: CHST4_HUMAN, HEC-GLCNAC-6-ST, LSST

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NCG5
Gene: CHST4
Ensembl: ENSG00000140835
Chromosome: 16
Canonical length: 386 aa
Protein class: Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]

Canonical amino-acid sequenceUniProt

386 residues, UniProt reviewed canonical sequence.

>Q8NCG5|CHST4
     1  MLLPKKMKLL LFLVSQMAIL ALFFHMYSHN ISSLSMKAQP ERMHVLVLSS WRSGSSFVGQ
    61  LFGQHPDVFY LMEPAWHVWM TFKQSTAWML HMAVRDLIRA VFLCDMSVFD AYMEPGPRRQ
   121  SSLFQWENSR ALCSAPACDI IPQDEIIPRA HCRLLCSQQP FEVVEKACRS YSHVVLKEVR
   181  FFNLQSLYPL LKDPSLNLHI VHLVRDPRAV FRSRERTKGD LMIDSRIVMG QHEQKLKKED
   241  QPYYVMQVIC QSQLEIYKTI QSLPKALQER YLLVRYEDLA RAPVAQTSRM YEFVGLEFLP
   301  HLQTWVHNIT RGKGMGDHAF HTNARDALNV SQAWRWSLPY EKVSRLQKAC GDAMNLLGYR
   361  HVRSEQEQRN LLLDLLSTWT VPEQIH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHST4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 65 nTPM

Expression across tissuesHPA

Tissue

gallbladder: 65 nTPM
pancreas: 12 nTPM
fallopian tube: 5 nTPM
liver: 4.1 nTPM
cervix: 2.3 nTPM
tonsil: 1.8 nTPM

Single-cell type

cholangiocytes: 269 nCPM
pancreatic duct cells: 30 nCPM
respiratory ciliated cells: 14 nCPM
fallopian secretory cells: 9.3 nCPM
epicardial cells: 9.1 nCPM
parietal cells: 8.8 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

choroid plexus: 4 nTPM
midbrain: 1.7 nTPM
medulla oblongata: 1.2 nTPM
pons: 1.2 nTPM
spinal cord: 0.9 nTPM
hippocampal formation: 0.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.65
gnomAD pLI: 0
gnomAD missense Z: 0.26
DepMap mean gene effect: -0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHST4 as an antibody target. Whether an autoantibody or antibody against CHST4 could matter depends on whether native CHST4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHST4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CHST4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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