Seroatlas · Human Serome Atlas

CD53

Leukocyte surface antigen CD53

Also known as: CD53_HUMAN, MOX44, TSPAN25

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P19397
Gene
CD53
Ensembl
ENSG00000143119
Chromosome
1
Canonical length
219 aa
Protein class
CD markers, Predicted membrane proteins, Transporters

OverviewNCBI Gene

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Canonical amino-acid sequenceUniProt

219 residues, UniProt reviewed canonical sequence.

>P19397|CD53
     1  MGMSSLKLLK YVLFFFNLLF WICGCCILGF GIYLLIHNNF GVLFHNLPSL TLGNVFVIVG
    61  SIIMVVAFLG CMGSIKENKC LLMSFFILLL IILLAEVTLA ILLFVYEQKL NEYVAKGLTD
   121  SIHRYHSDNS TKAAWDSIQS FLQCCGINGT SDWTSGPPAS CPSDRKVEGC YAKARLWFHS
   181  NFLYIGIITI CVCVIEVLGM SFALTLNCQI DKTSQTIGL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CD53 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
4
Mean surface accessibility (rSASA)
0.33
Highest tissue expression
420 nTPM

Expression across tissuesHPA

Tissue

  • tonsil: 420 nTPM
  • lymph node: 372 nTPM
  • appendix: 268 nTPM
  • spleen: 252 nTPM
  • thymus: 135 nTPM
  • bone marrow: 120 nTPM

Single-cell type

  • neutrophils: 2,135 nCPM
  • monocytes: 538 nCPM
  • hofbauer cells: 503 nCPM
  • nk-cells: 455 nCPM
  • b-cells: 454 nCPM
  • t-cells: 420 nCPM

Immune cell

  • total PBMC: 1,620 nTPM
  • basophil: 1,487 nTPM
  • neutrophil: 1,149 nTPM
  • non-classical monocyte: 887 nTPM
  • intermediate monocyte: 809 nTPM
  • memory B-cell: 797 nTPM

Brain region

  • white matter: 59 nTPM
  • medulla oblongata: 31 nTPM
  • spinal cord: 30 nTPM
  • thalamus: 29 nTPM
  • pons: 27 nTPM
  • cerebral cortex: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.99
gnomAD pLI
0
gnomAD missense Z
0.81
DepMap mean gene effect
-0.04
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CD53 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CD53 as an antibody target. Whether an autoantibody or antibody against CD53 could matter depends on whether native CD53 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CD53 is annotated at the cell surface, where native CD53 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label CD53 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CD53. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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