CD3D

Protein identityUniProt · HPA

UniProt accession

P04234

Gene

CD3D

Ensembl

ENSG00000167286

Chromosome

11

Canonical length

171 aa

Protein class

CD markers, Disease related genes, FDA approved drug targets, Human disease related genes, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Endoplasmic reticulum,Vesicles

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]

Canonical amino-acid sequenceUniProt

171 residues, UniProt reviewed canonical sequence.

>P04234|CD3D
     1  MEHSTFLSGL VLATLLSQVS PFKIPIEELE DRVFVNCNTS ITWVEGTVGT LLSDITRLDL
    61  GKRILDPRGI YRCNGTDIYK DKESTVQVHY RMCQSCVELD PATVAGIIVT DVIATLLLAL
   121  GVFCFAGHET GRLSGAADTQ ALLRNDQVYQ PLRDRDDAQY SHLGGNWARN K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CD3D can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.48

Highest tissue expression

809 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CD3D.

Disease | AllUniProt

Conditions CD3D is implicated in, by any mechanism.

• Immunodeficiency 19, severe combined (IMD19) MIM:615617

Disease | GeneticClinVar

17 pathogenic / likely-pathogenic of 229 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Immunodeficiency 19
• CD3D-related disorder
• Severe combined immunodeficiency disease

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.46

gnomAD pLI

0

gnomAD missense Z

0.01

DepMap mean gene effect

-0.13

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• adaptive immune response
• alpha-beta T cell activation
• cell surface receptor signaling pathway
• positive thymic T cell selection
• T cell receptor signaling pathway

Molecular functions

• identical protein binding
• transmembrane signaling receptor activity

Cellular components

• alpha-beta T cell receptor complex
• clathrin-coated endocytic vesicle membrane
• cytoplasm
• external side of plasma membrane
• plasma membrane
• T cell receptor complex

Protein domainsUniProt · Pfam · InterPro

• Phosphorylated immunoreceptor signalling ITAM
• Immunoglobulin-like fold
• CD3 protein, epsilon/gamma/delta subunit
• CD3 gamma/delta subunit, Ig-like domain
• Immunoglobulin-like domain superfamily
• Immunoreceptor tyrosine-based activation motif
• T-cell surface glycoprotein CD3 delta chain

KeywordsUniProt

• Adaptive immunity
• Cell membrane
• Disulfide bond
• Glycoprotein
• Immunity
• Membrane
• Phosphoprotein
• Receptor
• SCID
• Signal
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of CD3D in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CD8
• CD8B