CD14

Protein identityUniProt · HPA

UniProt accession

P08571

Gene

CD14

Ensembl

ENSG00000170458

Chromosome

5

Canonical length

375 aa

Protein class

Cancer-related genes, CD markers, Human disease related genes, Plasma proteins, Predicted secreted proteins, Transporters

Subcellular location

Vesicles

Secretome location

Secreted to blood

OverviewNCBI Gene

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2020]

Canonical amino-acid sequenceUniProt

375 residues, UniProt reviewed canonical sequence.

>P08571|CD14
     1  MERASCLLLL LLPLVHVSAT TPEPCELDDE DFRCVCNFSE PQPDWSEAFQ CVSAVEVEIH
    61  AGGLNLEPFL KRVDADADPR QYADTVKALR VRRLTVGAAQ VPAQLLVGAL RVLAYSRLKE
   121  LTLEDLKITG TMPPLPLEAT GLALSSLRLR NVSWATGRSW LAELQQWLKP GLKVLSIAQA
   181  HSPAFSCEQV RAFPALTSLD LSDNPGLGER GLMAALCPHK FPAIQNLALR NTGMETPTGV
   241  CAALAAAGVQ PHSLDLSHNS LRATVNPSAP RCMWSSALNS LNLSFAGLEQ VPKGLPAKLR
   301  VLDLSCNRLN RAPQPDELPE VDNLTLDGNP FLVPGTALPH EGSMNSGVVP ACARSTLSVG
   361  VSGTLVLLQG ARGFA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CD14 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

426 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for CD14 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.59

gnomAD pLI

0

gnomAD missense Z

0.55

DepMap mean gene effect

-0.06

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic process
• cell surface pattern recognition receptor signaling pathway
• cell surface receptor signaling pathway
• cellular response to diacyl bacterial lipopeptide
• cellular response to lipopolysaccharide
• cellular response to lipoteichoic acid
• cellular response to molecule of bacterial origin
• cellular response to triacyl bacterial lipopeptide
• inflammatory response
• innate immune response
• phagocytosis
• positive regulation of cytokine production
• positive regulation of endocytosis
• positive regulation of interleukin-8 production
• positive regulation of lipopolysaccharide-mediated signaling pathway
• positive regulation of toll-like receptor 4 signaling pathway
• positive regulation of tumor necrosis factor production
• positive regulation of type I interferon production
• positive regulation of type II interferon production
• receptor-mediated endocytosis
• toll-like receptor 4 signaling pathway

Molecular functions

• lipopolysaccharide binding
• lipopolysaccharide immune receptor activity
• lipoteichoic acid binding
• molecular carrier activity
• peptidoglycan immune receptor activity
• opsonin receptor activity

Cellular components

• endosome membrane
• external side of plasma membrane
• extracellular exosome
• extracellular region
• extracellular space
• Golgi apparatus
• lipopolysaccharide receptor complex
• membrane raft
• plasma membrane
• secretory granule membrane

Protein domainsUniProt · Pfam · InterPro

• Leucine-rich repeat
• Leucine-rich repeat domain superfamily
• Leucine Rich repeat
• Monocyte differentiation antigen CD14

KeywordsUniProt

• Cell membrane
• Disulfide bond
• Glycoprotein
• Golgi apparatus
• GPI-anchor
• Immunity
• Inflammatory response
• Innate immunity
• Leucine-rich repeat
• Lipoprotein
• Membrane
• Repeat
• Secreted
• Signal

InteractionsUniProt · HPA

Protein binding partners of CD14 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• FSTL1
• LPAR1
• MYO18A
• TLR6