CASP5

Protein identityUniProt · HPA

UniProt accession

P51878

Gene

CASP5

Ensembl

ENSG00000137757

Chromosome

11

Canonical length

434 aa

Protein class

Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Canonical amino-acid sequenceUniProt

434 residues, UniProt reviewed canonical sequence.

>P51878|CASP5
     1  MAEDSGKKKR RKNFEAMFKG ILQSGLDNFV INHMLKNNVA GQTSIQTLVP NTDQKSTSVK
    61  KDNHKKKTVK MLEYLGKDVL HGVFNYLAKH DVLTLKEEEK KKYYDTKIED KALILVDSLR
   121  KNRVAHQMFT QTLLNMDQKI TSVKPLLQIE AGPPESAEST NILKLCPREE FLRLCKKNHD
   181  EIYPIKKRED RRRLALIICN TKFDHLPARN GAHYDIVGMK RLLQGLGYTV VDEKNLTARD
   241  MESVLRAFAA RPEHKSSDST FLVLMSHGIL EGICGTAHKK KKPDVLLYDT IFQIFNNRNC
   301  LSLKDKPKVI IVQACRGEKH GELWVRDSPA SLALISSQSS ENLEADSVCK IHEEKDFIAF
   361  CSSTPHNVSW RDRTRGSIFI TELITCFQKY SCCCHLMEIF RKVQKSFEVP QAKAQMPTIE
   421  RATLTRDFYL FPGN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CASP5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

16 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.55

gnomAD pLI

0

gnomAD missense Z

0.47

DepMap mean gene effect

0.09

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic process
• cellular response to mechanical stimulus
• positive regulation of inflammatory response
• positive regulation of neuron apoptotic process
• protein maturation
• proteolysis
• substantia nigra development

Molecular functions

• cysteine-type endopeptidase activity
• cysteine-type peptidase activity

Cellular components

• cytoplasm
• cytosol
• NLRP1 inflammasome complex

Protein domainsUniProt · Pfam · InterPro

• Peptidase C14, p20 domain
• CARD domain
• Peptidase C14, caspase non-catalytic subunit p10
• Peptidase C14 family
• Death-like domain superfamily
• Peptidase C14, caspase domain
• Peptidase C14A, caspase catalytic domain
• Peptidase family C14A, His active site
• Caspase-like domain superfamily
• Peptidase family C14A, cysteine active site
• Caspase recruitment domain
• Caspase domain

KeywordsUniProt

• Alternative initiation
• Apoptosis
• Hydrolase
• Protease
• Thiol protease
• Zymogen

InteractionsUniProt · HPA

Protein binding partners of CASP5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MEFV
• SERPINB1
• NLRC3