CAPN2

Protein identityUniProt · HPA

UniProt accession

P17655

Gene

CAPN2

Ensembl

ENSG00000162909

Chromosome

1

Canonical length

700 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol,Connecting piece,Mid piece,Principal piece

OverviewNCBI Gene

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Canonical amino-acid sequenceUniProt

700 residues, UniProt reviewed canonical sequence.

>P17655|CAPN2
     1  MAGIAAKLAK DREAAEGLGS HDRAIKYLNQ DYEALRNECL EAGTLFQDPS FPAIPSALGF
    61  KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG ALGDCWLLAA IASLTLNEEI
   121  LARVVPLNQS FQENYAGIFH FQFWQYGEWV EVVVDDRLPT KDGELLFVHS AEGSEFWSAL
   181  LEKAYAKING CYEALSGGAT TEGFEDFTGG IAEWYELKKP PPNLFKIIQK ALQKGSLLGC
   241  SIDITSAADS EAITFQKLVK GHAYSVTGAE EVESNGSLQK LIRIRNPWGE VEWTGRWNDN
   301  CPSWNTIDPE ERERLTRRHE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTS DTYKKWKLTK
   361  MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED GESGCTFLVG LIQKHRRRQR
   421  KMGEDMHTIG FGIYEVPEEL SGQTNIHLSK NFFLTNRARE RSDTFINLRE VLNRFKLPPG
   481  EYILVPSTFE PNKDGDFCIR VFSEKKADYQ AVDDEIEANL EEFDISEDDI DDGFRRLFAQ
   541  LAGEDAEISA FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL
   601  WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF ADDQLIIDFD
   661  NFVRCLVRLE TLFKIFKQLD PENTGTIELD LISWLCFSVL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CAPN2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.25

Highest tissue expression

172 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.07

gnomAD pLI

0

gnomAD missense Z

0.59

DepMap mean gene effect

-0.01

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• behavioral response to pain
• blastocyst development
• cellular response to amino acid stimulus
• cellular response to interferon-beta
• cellular response to lipopolysaccharide
• female pregnancy
• myoblast fusion
• positive regulation of cardiac muscle cell apoptotic process
• positive regulation of myoblast fusion
• positive regulation of phosphatidylcholine biosynthetic process
• protein autoprocessing
• protein catabolic process at postsynapse
• proteolysis
• proteolysis involved in protein catabolic process
• regulation of cytoskeleton organization
• regulation of interleukin-6 production
• response to hydrogen peroxide
• response to hypoxia
• response to mechanical stimulus
• synaptic vesicle endocytosis
• vascular endothelial cell response to laminar fluid shear stress
• vascular endothelial cell response to oscillatory fluid shear stress

Molecular functions

• calcium ion binding
• calcium-dependent cysteine-type endopeptidase activity
• cysteine-type peptidase activity
• cytoskeletal protein binding
• enzyme binding
• protein-containing complex binding

Cellular components

• calpain complex
• chromatin
• cortical actin cytoskeleton
• cytoplasm
• cytosol
• dendrite
• endoplasmic reticulum
• external side of plasma membrane
• extracellular exosome
• focal adhesion
• Golgi apparatus
• lysosome
• membrane raft
• neuronal cell body
• nucleus
• perinuclear endoplasmic reticulum
• plasma membrane
• postsynapse
• presynapse
• pseudopodium
• sperm head-tail coupling apparatus
• sperm midpiece
• sperm principal piece

Protein domainsUniProt · Pfam · InterPro

• Cysteine peptidase, cysteine active site
• Peptidase C2, calpain, catalytic domain
• EF-hand domain
• EF-hand domain pair
• EF-Hand 1, calcium-binding site
• Peptidase C2, calpain, large subunit, domain III
• Peptidase C2, calpain, domain III
• Peptidase C2, calpain family
• Calpain subdomain III
• Calpain large subunit, domain III superfamily
• Papain-like cysteine peptidase superfamily
• Calpain family cysteine protease
• Calpain large subunit, domain III
• EF-hand domain pair
• CAPN2, penta-EF hand, calcium binding motif

KeywordsUniProt

• Acetylation
• Calcium
• Cell membrane
• Cytoplasm
• Hydrolase
• Membrane
• Metal-binding
• Protease
• Repeat
• Thiol protease

InteractionsUniProt · HPA

Protein binding partners of CAPN2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CPEB3
• CAPNS1
• ZDHHC17