Seroatlas · Human Serome Atlas

BROX

BRO1 domain-containing protein BROX

Also known as: BROX_HUMAN, C1orf58, FLJ32421

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5VW32
Gene: BROX
Ensembl: ENSG00000162819
Chromosome: 1
Canonical length: 411 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Golgi apparatus,Cytosol

OverviewNCBI Gene

Involved in mitotic nuclear membrane reassembly. Located in nuclear envelope. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

411 residues, UniProt reviewed canonical sequence.

>Q5VW32|BROX
     1  MTHWFHRNPL KATAPVSFNY YGVVTGPSAS KICNDLRSSR ARLLELFTDL SCNPEMMKNA
    61  ADSYFSLLQG FINSLDESTQ ESKLRYIQNF KWTDTLQGQV PSAQQDAVFE LISMGFNVAL
   121  WYTKYASRLA GKENITEDEA KEVHRSLKIA AGIFKHLKES HLPKLITPAE KGRDLESRLI
   181  EAYVIQCQAE AQEVTIARAI ELKHAPGLIA ALAYETANFY QKADHTLSSL EPAYSAKWRK
   241  YLHLKMCFYT AYAYCYHGET LLASDKCGEA IRSLQEAEKL YAKAEALCKE YGETKGPGPT
   301  VKPSGHLFFR KLGNLVKNTL EKCQRENGFI YFQKIPTEAP QLELKANYGL VEPIPFEFPP
   361  TSVQWTPETL AAFDLTKRPK DDSTKPKPEE EVKPVKEPDI KPQKDTGCYI S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BROX can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.24
Highest tissue expression: 39 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 39 nTPM
esophagus: 26 nTPM
small intestine: 26 nTPM
skeletal muscle: 25 nTPM
thyroid gland: 25 nTPM
parathyroid gland: 25 nTPM

Single-cell type

esophageal apical cells: 224 nCPM
platelets: 150 nCPM
neutrophils: 124 nCPM
microglia: 95 nCPM
kupffer cells: 85 nCPM
gastric progenitor cells: 78 nCPM

Immune cell

basophil: 76 nTPM
neutrophil: 53 nTPM
non-classical monocyte: 44 nTPM
intermediate monocyte: 42 nTPM
eosinophil: 37 nTPM
NK-cell: 28 nTPM

Brain region

white matter: 73 nTPM
medulla oblongata: 55 nTPM
spinal cord: 52 nTPM
basal ganglia: 49 nTPM
hypothalamus: 49 nTPM
cerebellum: 49 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.06
gnomAD pLI: 0
gnomAD missense Z: 0.38
DepMap mean gene effect: 0.11
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

mitotic nuclear membrane reassembly

Cellular components

Protein domainsUniProt · Pfam · InterPro

BRO1 domain
BRO1 domain superfamily
BRO1-like domain
BRO1 domain-containing protein BROX

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of BROX in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads BROX as an antibody target. Whether an autoantibody or antibody against BROX could matter depends on whether native BROX is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

BROX is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label BROX as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...