Seroatlas · Human Serome Atlas

B4GALT5

Beta-1,4-galactosyltransferase 5

Also known as: B4GT5_HUMAN, beta4GalT-V

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O43286
Gene
B4GALT5
Ensembl
ENSG00000158470
Chromosome
20
Canonical length
388 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Vesicles

OverviewNCBI Gene

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

388 residues, UniProt reviewed canonical sequence.

>O43286|B4GALT5
     1  MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMMQAQG ILIRDNVRTI
    61  GAQVYEQVLR SAYAKRNSSV NDSDYPLDLN HSETFLQTTT FLPEDFTYFA NHTCPERLPS
   121  MKGPIDINMS EIGMDYIHEL FSKDPTIKLG GHWKPSDCMP RWKVAILIPF RNRHEHLPVL
   181  FRHLLPMLQR QRLQFAFYVV EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE
   241  SDRNYYGCGQ MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED
   301  DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER QGLDGLNNLN
   361  YFANITYDAL YKNITVNLTP ELAQVNEY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against B4GALT5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.29
Highest tissue expression
89 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 89 nTPM
  • skeletal muscle: 63 nTPM
  • urinary bladder: 48 nTPM
  • adrenal gland: 45 nTPM
  • esophagus: 36 nTPM
  • smooth muscle: 35 nTPM

Single-cell type

  • neutrophil progenitors: 1,671 nCPM
  • neutrophils: 1,512 nCPM
  • urothelial cells: 734 nCPM
  • ocular epithelial cells: 682 nCPM
  • mast cells: 674 nCPM
  • salivary duct cells: 496 nCPM

Immune cell

  • neutrophil: 6.6 nTPM
  • eosinophil: 2.6 nTPM
  • MAIT T-cell: 1.5 nTPM
  • basophil: 1.4 nTPM
  • gdT-cell: 1.4 nTPM
  • memory CD8 T-cell: 1.3 nTPM

Brain region

  • midbrain: 45 nTPM
  • hypothalamus: 43 nTPM
  • pons: 37 nTPM
  • cerebral cortex: 37 nTPM
  • thalamus: 36 nTPM
  • medulla oblongata: 35 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.15
gnomAD pLI
1
gnomAD missense Z
2.37
DepMap mean gene effect
-0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads B4GALT5 as an antibody target. Whether an autoantibody or antibody against B4GALT5 could matter depends on whether native B4GALT5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

B4GALT5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label B4GALT5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/B4GALT5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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