Seroatlas · Human Serome Atlas

B4GALT2

Beta-1,4-galactosyltransferase 2

Also known as: B4GT2_HUMAN, beta4Gal-T2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O60909
Gene
B4GALT2
Ensembl
ENSG00000117411
Chromosome
1
Canonical length
372 aa
Protein class
Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location
Golgi apparatus,Vesicles

OverviewNCBI Gene

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Canonical amino-acid sequenceUniProt

372 residues, UniProt reviewed canonical sequence.

>O60909|B4GALT2
     1  MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSARGP AHALHPAASS
    61  SSSSSNCSRP NATASSSGLP EVPSALPGPT APTLPPCPDS PPGLVGRLLI EFTSPMPLER
   121  VQRENPGVLM GGRYTPPDCT PAQTVAVIIP FRHREHHLRY WLHYLHPILR RQRLRYGVYV
   181  INQHGEDTFN RAKLLNVGFL EALKEDAAYD CFIFSDVDLV PMDDRNLYRC GDQPRHFAIA
   241  MDKFGFRLPY AGYFGGVSGL SKAQFLRING FPNEYWGWGG EDDDIFNRIS LTGMKISRPD
   301  IRIGRYRMIK HDRDKHNEPN PQRFTKIQNT KLTMKRDGIG SVRYQVLEVS RQPLFTNITV
   361  DIGRPPSWPP RG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against B4GALT2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.34
Highest tissue expression
66 nTPM

Expression across tissuesHPA

Tissue

  • amygdala: 66 nTPM
  • cerebral cortex: 63 nTPM
  • hippocampal formation: 53 nTPM
  • skeletal muscle: 49 nTPM
  • basal ganglia: 42 nTPM
  • pancreas: 40 nTPM

Single-cell type

  • alveolar cells type 1: 42 nCPM
  • pancreatic acinar cells: 38 nCPM
  • esophageal basal cells: 28 nCPM
  • breast lactating cells: 28 nCPM
  • alveolar cells type 2: 25 nCPM
  • ocular epithelial cells: 25 nCPM

Immune cell

  • MAIT T-cell: 0.6 nTPM
  • memory B-cell: 0.2 nTPM
  • memory CD8 T-cell: 0.2 nTPM
  • memory CD4 T-cell: 0.1 nTPM
  • naive B-cell: 0.1 nTPM
  • naive CD8 T-cell: 0.1 nTPM

Brain region

  • amygdala: 63 nTPM
  • hippocampal formation: 57 nTPM
  • thalamus: 52 nTPM
  • cerebral cortex: 51 nTPM
  • midbrain: 49 nTPM
  • pons: 47 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.59
gnomAD pLI
0.2
gnomAD missense Z
1.88
DepMap mean gene effect
-0.11
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads B4GALT2 as an antibody target. Whether an autoantibody or antibody against B4GALT2 could matter depends on whether native B4GALT2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

B4GALT2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label B4GALT2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/B4GALT2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...