ASCL4
Achaete-scute homolog 4
Also known as: ASCL4_HUMAN, bHLHa44, HASH4
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q6XD76
- Gene
- ASCL4
- Ensembl
- ENSG00000187855
- Chromosome
- 12
- Canonical length
- 172 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
OverviewNCBI Gene
Basic helix-loop-helix transcription factors, such as ASCL4, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM, Mar 2008]
Canonical amino-acid sequenceUniProt
172 residues, UniProt reviewed canonical sequence.
>Q6XD76|ASCL4
1 METRKPAERL ALPYSLRTAP LGVPGTLPGL PRRDPLRVAL RLDAACWEWA RSGCARGWQY
61 LPVPLDSAFE PAFLRKRNER ERQRVRCVNE GYARLRDHLP RELADKRLSK VETLRAAIDY
121 IKHLQELLER QAWGLEGAAG AVPQRRAECN SDGESKASSA PSPSSEPEEG GSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against ASCL4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.63
- Highest tissue expression
- 0.2 nTPM
Expression across tissuesHPA
Tissue
- tonsil: 0.2 nTPM
- kidney: 0.1 nTPM
- lymph node: 0.1 nTPM
- parathyroid gland: 0.1 nTPM
- skin: 0.1 nTPM
- thymus: 0.1 nTPM
Single-cell type
- microglia: 9.9 nCPM
- respiratory ionocytes: 5.1 nCPM
- retinal ganglion cells: 3.9 nCPM
- epicardial cells: 2.3 nCPM
- cdc: 2.2 nCPM
- macrophages: 0.9 nCPM
Immune cell
- myeloid DC: 0.5 nTPM
- plasmacytoid DC: 0.1 nTPM
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
Brain region
- pons: 0.1 nTPM
- amygdala: 0 nTPM
- basal ganglia: 0 nTPM
- cerebellum: 0 nTPM
- cerebral cortex: 0 nTPM
- choroid plexus: 0 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.83
- gnomAD pLI
- 0
- gnomAD missense Z
- -1.04
- DepMap mean gene effect
- -0.07
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
- DNA-binding transcription factor activity, RNA polymerase II-specific
- DNA-binding transcription repressor activity, RNA polymerase II-specific
- protein dimerization activity
- RNA polymerase II transcription regulatory region sequence-specific DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of ASCL4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads ASCL4 as an antibody target. Whether an autoantibody or antibody against ASCL4 could matter depends on whether native ASCL4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
ASCL4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label ASCL4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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