ARHGAP5

Protein identityUniProt · HPA

UniProt accession

Q13017

Gene

ARHGAP5

Ensembl

ENSG00000100852

Chromosome

14

Canonical length

1502 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1502 residues, UniProt reviewed canonical sequence.

>Q13017|ARHGAP5
     1  MMAKNKEPRP PSYTISIVGL SGTEKDKGNC GVGKSCLCNR FVRSKADEYY PEHTSVLSTI
    61  DFGGRVVNND HFLYWGDIIQ NSEDGVECKI HVIEQTEFID DQTFLPHRST NLQPYIKRAA
   121  ASKLQSAEKL MYICTDQLGL EQDFEQKQMP EGKLNVDGFL LCIDVSQGCN RKFDDQLKFV
   181  NNLFVQLSKS KKPVIIAATK CDECVDHYLR EVQAFASNKK NLLVVETSAR FNVNIETCFT
   241  ALVQMLDKTR SKPKIIPYLD AYKTQRQLVV TATDKFEKLV QTVRDYHATW KTVSNKLKNH
   301  PDYEEYINLE GTRKARNTFS KHIEQLKQEH IRKRREEYIN TLPRAFNTLL PNLEEIEHLN
   361  WSEALKLMEK RADFQLCFVV LEKTPWDETD HIDKINDRRI PFDLLSTLEA EKVYQNHVQH
   421  LISEKRRVEM KEKFKKTLEK IQFISPGQPW EEVMCFVMED EAYKYITEAD SKEVYGRHQR
   481  EIVEKAKEEF QEMLFEHSEL FYDLDLNATP SSDKMSEIHT VLSEEPRYKA LQKLAPDRES
   541  LLLKHIGFVY HPTKETCLSG QNCTDIKVEQ LLASSLLQLD HGRLRLYHDS TNIDKVNLFI
   601  LGKDGLAQEL ANEIRTQSTD DEYALDGKIY ELDLRPVDAK SPYFLSQLWT AAFKPHGCFC
   661  VFNSIESLSF IGEFIGKIRT EASQIRKDKY MANLPFTLIL ANQRDSISKN LPILRHQGQQ
   721  LANKLQCPFV DVPAGTYPRK FNETQIKQAL RGVLESVKHN LDVVSPIPAN KDLSEADLRI
   781  VMCAMCGDPF SVDLILSPFL DSHSCSAAQA GQNNSLMLDK IIGEKRRRIQ ITILSYHSSI
   841  GVRKDELVHG YILVYSAKRK ASMGMLRAFL SEVQDTIPVQ LVAVTDSQAD FFENEAIKEL
   901  MTEGEHIATE ITAKFTALYS LSQYHRQTEV FTLFFSDVLE KKNMIENSYL SDNTRESTHQ
   961  SEDVFLPSPR DCFPYNNYPD SDDDTEAPPP YSPIGDDVQL LPTPSDRSRY RLDLEGNEYP
  1021  IHSTPNCHDH ERNHKVPPPI KPKPVVPKTN VKKLDPNLLK TIEAGIGKNP RKQTSRVPLA
  1081  HPEDMDPSDN YAEPIDTIFK QKGYSDEIYV VPDDSQNRIK IRNSFVNNTQ GDEENGFSDR
  1141  TSKSHGERRP SKYKYKSKTL FSKAKSYYRR THSDASDDEA FTTSKTKRKG RHRGSEEDPL
  1201  LSPVETWKGG IDNPAITSDQ ELDDKKMKKK THKVKEDKKQ KKKTKNFNPP TRRNWESNYF
  1261  GMPLQDLVTA EKPIPLFVEK CVEFIEDTGL CTEGLYRVSG NKTDQDNIQK QFDQDHNINL
  1321  VSMEVTVNAV AGALKAFFAD LPDPLIPYSL HPELLEAAKI PDKTERLHAL KEIVKKFHPV
  1381  NYDVFRYVIT HLNRVSQQHK INLMTADNLS ICFWPTLMRP DFENREFLST TKIHQSVVET
  1441  FIQQCQFFFY NGEIVETTNI VAPPPPSNPG QLVEPMVPLQ LPPPLQPQLI QPQLQTDPLG
  1501  II

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGAP5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.37

Highest tissue expression

64 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ARHGAP5.

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 167 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Martsolf syndrome 1
• Pulmonary artery atresia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.26

gnomAD pLI

1

gnomAD missense Z

2.21

DepMap mean gene effect

-0.11

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell adhesion
• epithelial cell migration
• mammary gland development
• positive regulation of epithelial cell migration
• positive regulation of mesenchymal cell proliferation
• regulation of cell size
• regulation of small GTPase mediated signal transduction
• Rho protein signal transduction

Molecular functions

• GTPase activator activity
• GTPase activity
• SH2 domain binding

Cellular components

• cytoplasm
• cytosol
• endoplasmic reticulum
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Rho GTPase-activating protein domain
• FF domain
• Rho GTPase activation protein
• P-loop containing nucleoside triphosphate hydrolase
• Rho GTPase-activating protein, FF domain
• FF domain superfamily
• Rho GTPase-activating protein, pG2 domain
• Rho GTPase-activating protein, pG1 domain
• Rho GTPase-activating protein, pG1 and pG2 domain
• Rho GTPase-activating domain-containing protein
• Rho GTPase-activating protein 35-like, FF domain
• RhoGAP domain
• FF domain
• p190-A and -B Rho GAPs FF domain
• p190RhoGAP, pG1 and pG2 domains
• Rho GTPase-activating protein 35-like, FF domain

KeywordsUniProt

• Cell membrane
• Cytoplasm
• GTPase activation
• Membrane
• Nitration
• Phosphoprotein
• Repeat

InteractionsUniProt · HPA

Protein binding partners of ARHGAP5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SMURF2