ARHGAP35

Protein identityUniProt · HPA

UniProt accession

Q9NRY4

Gene

ARHGAP35

Ensembl

ENSG00000160007

Chromosome

19

Canonical length

1499 aa

Protein class

Cancer-related genes, Predicted intracellular proteins

Subcellular location

Nuclear bodies,Microtubules,Aggresome

OverviewNCBI Gene

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1499 residues, UniProt reviewed canonical sequence.

>Q9NRY4|ARHGAP35
     1  MMMARKQDVR IPTYNISVVG LSGTEKEKGQ CGIGKSCLCN RFVRPSADEF HLDHTSVLST
    61  SDFGGRVVNN DHFLYWGEVS RSLEDCVECK MHIVEQTEFI DDQTFQPHRS TALQPYIKRA
   121  AATKLASAEK LMYFCTDQLG LEQDFEQKQM PDGKLLVDGF LLGIDVSRGM NRNFDDQLKF
   181  VSNLYNQLAK TKKPIVVVLT KCDEGVERYI RDAHTFALSK KNLQVVETSA RSNVNVDLAF
   241  STLVQLIDKS RGKTKIIPYF EALKQQSQQI ATAKDKYEWL VSRIVKNHNE NWLSVSRKMQ
   301  ASPEYQDYVY LEGTQKAKKL FLQHIHRLKH EHIERRRKLY LAALPLAFEA LIPNLDEIDH
   361  LSCIKAKKLL ETKPEFLKWF VVLEETPWDA TSHIDNMENE RIPFDLMDTV PAEQLYEAHL
   421  EKLRNERKRV EMRRAFKENL ETSPFITPGK PWEEARSFIM NEDFYQWLEE SVYMDIYGKH
   481  QKQIIDKAKE EFQELLLEYS ELFYELELDA KPSKEKMGVI QDVLGEEQRF KALQKLQAER
   541  DALILKHIHF VYHPTKETCP SCPACVDAKI EHLISSRFIR PSDRNQKNSL SDPNIDRINL
   601  VILGKDGLAR ELANEIRALC TNDDKYVIDG KMYELSLRPI EGNVRLPVNS FQTPTFQPHG
   661  CLCLYNSKES LSYVVESIEK SRESTLGRRD NHLVHLPLTL ILVNKRGDTS GETLHSLIQQ
   721  GQQIASKLQC VFLDPASAGI GYGRNINEKQ ISQVLKGLLD SKRNLNLVSS TASIKDLADV
   781  DLRIVMCLMC GDPFSADDIL FPVLQSQTCK SSHCGSNNSV LLELPIGLHK KRIELSVLSY
   841  HSSFSIRKSR LVHGYIVFYS AKRKASLAML RAFLCEVQDI IPIQLVALTD GAVDVLDNDL
   901  SREQLTEGEE IAQEIDGRFT SIPCSQPQHK LEIFHPFFKD VVEKKNIIEA THMYDNAAEA
   961  CSTTEEVFNS PRAGSPLCNS NLQDSEEDIE PSYSLFREDT SLPSLSKDHS KLSMELEGND
  1021  GLSFIMSNFE SKLNNKVPPP VKPKPPVHFE ITKGDLSYLD QGHRDGQRKS VSSSPWLPQD
  1081  GFDPSDYAEP MDAVVKPRNE EENIYSVPHD STQGKIITIR NINKAQSNGS GNGSDSEMDT
  1141  SSLERGRKVS IVSKPVLYRT RCTRLGRFAS YRTSFSVGSD DELGPIRKKE EDQASQGYKG
  1201  DNAVIPYETD EDPRRRNILR SLRRNTKKPK PKPRPSITKA TWESNYFGVP LTTVVTPEKP
  1261  IPIFIERCIE YIEATGLSTE GIYRVSGNKS EMESLQRQFD QDHNLDLAEK DFTVNTVAGA
  1321  MKSFFSELPD PLVPYNMQID LVEAHKINDR EQKLHALKEV LKKFPKENHE VFKYVISHLN
  1381  KVSHNNKVNL MTSENLSICF WPTLMRPDFS TMDALTATRT YQTIIELFIQ QCPFFFYNRP
  1441  ITEPPGARPS SPSAVASTVP FLTSTPVTSQ PSPPQSPPPT PQSPMQPLLP SQLQAEHTL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGAP35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.37

Highest tissue expression

33 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ARHGAP35.

Disease | GeneticClinVar

20 pathogenic / likely-pathogenic of 173 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Martsolf syndrome 1
• Neurodevelopmental disorder
• Unilateral microphthalmos
• Irido-corneo-trabecular dysgenesis
• Bilateral microphthalmos

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.06

gnomAD pLI

1

gnomAD missense Z

4.16

DepMap mean gene effect

0.18

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• axon guidance
• axonal fasciculation
• camera-type eye development
• cell migration
• central nervous system neuron axonogenesis
• establishment or maintenance of actin cytoskeleton polarity
• forebrain development
• mammary gland development
• negative regulation of Rho protein signal transduction
• negative regulation of vascular permeability
• neural tube closure
• neuron projection guidance
• positive regulation of cilium assembly
• positive regulation of neuron projection development
• regulation of actin cytoskeleton organization
• regulation of actin polymerization or depolymerization
• regulation of axonogenesis
• regulation of cell shape
• regulation of cell size
• regulation of small GTPase mediated signal transduction
• Rho protein signal transduction
• wound healing, spreading of cells

Molecular functions

• DNA binding
• GTP binding
• GTPase activator activity
• GTPase activity
• phospholipid binding

Cellular components

• actin cytoskeleton
• ciliary basal body
• cytosol
• nucleus
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Rho GTPase-activating protein domain
• Small GTPase
• FF domain
• Rho GTPase activation protein
• P-loop containing nucleoside triphosphate hydrolase
• Rho GTPase-activating protein, FF domain
• FF domain superfamily
• Rho GTPase-activating protein, pG2 domain
• Rho GTPase-activating protein, pG1 domain
• Rho GTPase-activating protein, pG1 and pG2 domain
• Rho GTPase-activating domain-containing protein
• Rho GTPase-activating protein 35-like, FF domain
• Ras family
• RhoGAP domain
• p190-A and -B Rho GAPs FF domain
• p190RhoGAP, pG1 and pG2 domains
• Rho GTPase-activating protein 35-like, FF domain

KeywordsUniProt

• Cell membrane
• Cell projection
• Cytoplasm
• Cytoskeleton
• DNA-binding
• GTP-binding
• GTPase activation
• Lipid-binding
• Membrane
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Repeat
• Repressor
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of ARHGAP35 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RASA1