Seroatlas · Human Serome Atlas

ARAP3

Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3

Also known as: ARAP3_HUMAN, CENTD3, DRAG1, FLJ21065

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WWN8
Gene: ARAP3
Ensembl: ENSG00000120318
Chromosome: 5
Canonical length: 1544 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Sep 2015]

Canonical amino-acid sequenceUniProt

1544 residues, UniProt reviewed canonical sequence.

>Q8WWN8|ARAP3
     1  MAAPQDLDIA VWLATVHLEQ YADTFRRHGL ATAGAARGLG HEELKQLGIS ATGHRKRILR
    61  LLQTGTEEGS LDPKSDSAME PSPSPAPQAQ PPKPVPKPRT VFGGLSGPAT TQRPGLSPAL
   121  GGPGVSRSPE PSPRPPPLPT SSSEQSSALN TVEMMPNSIY FGLDSRGRAQ AAQDKAPDSS
   181  QISAPTPALR PTTGTVHIMD PGCLYYGVQP VGTPGAPDRR ESRGVCQGRA EHRLSRQDLE
   241  AREDAGYASL ELPGDSTLLS PTLETEETSD DLISPYASFS FTADRLTPLL SGWLDKLSPQ
   301  GNYVFQRRFV QFNGRSLMYF GSDKDPFPKG VIPLTAIEMT RSSKDNKFQV ITGQRVFVFR
   361  TESEAQRDMW CSTLQSCLKE QRLLGHPRPP QPPRPLRTGM LELRGHKAKV FAALSPGELA
   421  LYKSEQAFSL GIGICFIELQ GCSVRETKSR SFDLLTPHRC FSFTAESGGA RQSWAAALQE
   481  AVTETLSDYE VAEKIWSNRA NRQCADCGSS RPDWAAVNLG VVICKQCAGQ HRALGSGISK
   541  VQSLKLDTSV WSNEIVQLFI VLGNDRANRF WAGTLPPGEG LHPDATPGPR GEFISRKYRL
   601  GLFRKPHPQY PDHSQLLQAL CAAVARPNLL KNMTQLLCVE AFEGEEPWFP PAPDGSCPGL
   661  LPSDPSPGVY NEVVVRATYS GFLYCSPVSN KAGPSPPRRG RDAPPRLWCV LGAALEMFAS
   721  ENSPEPLSLI QPQDIVCLGV SPPPTDPGDR FPFSFELILA GGRIQHFGTD GADSLEAWTS
   781  AVGKWFSPLS CHQLLGPGLL RLGRLWLRSP SHTAPAPGLW LSGFGLLRGD HLFLCSAPGP
   841  GPPAPEDMVH LRRLQEISVV SAADTPDKKE HLVLVETGRT LYLQGEGRLD FTAWNAAIGG
   901  AAGGGGTGLQ EQQMSRGDIP IIVDACISFV TQHGLRLEGV YRKGGARARS LRLLAEFRRD
   961  ARSVKLRPGE HFVEDVTDTL KRFFRELDDP VTSARLLPRW REAAELPQKN QRLEKYKDVI
  1021  GCLPRVNRRT LATLIGHLYR VQKCAALNQM CTRNLALLFA PSVFQTDGRG EHEVRVLQEL
  1081  IDGYISVFDI DSDQVAQIDL EVSLITTWKD VQLSQAGDLI MEVYIEQQLP DNCVTLKVSP
  1141  TLTAEELTNQ VLEMRGTAAG MDLWVTFEIR EHGELERPLH PKEKVLEQAL QWCQLPEPCS
  1201  ASLLLKKVPL AQAGCLFTGI RRESPRVGLL RCREEPPRLL GSRFQERFFL LRGRCLLLLK
  1261  EKKSSKPERE WPLEGAKVYL GIRKKLKPPT PWGFTLILEK MHLYLSCTDE DEMWDWTTSI
  1321  LKAQHDDQQP VVLRRHSSSD LARQKFGTMP LLPIRGDDSG ATLLSANQTL RRLHNRRTLS
  1381  MFFPMKSSQG SVEEQEELEE PVYEEPVYEE VGAFPELIQD TSTSFSTTRE WTVKPENPLT
  1441  SQKSLDQPFL SKSSTLGQEE RPPEPPPGPP SKSSPQARGS LEEQLLQELS SLILRKGETT
  1501  AGLGSPSQPS SPQSPSPTGL PTQTPGFPTQ PPCTSSPPSS QPLT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARAP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.4
Highest tissue expression: 15 nTPM

Expression across tissuesHPA

Tissue

lung: 15 nTPM
heart muscle: 14 nTPM
placenta: 11 nTPM
adipose tissue: 11 nTPM
breast: 9.5 nTPM
cervix: 8.6 nTPM

Single-cell type

neutrophils: 133 nCPM
retinal horizontal cells: 128 nCPM
vascular endothelial cells: 89 nCPM
lymphatic endothelial cells: 63 nCPM
prostatic hillock cells: 30 nCPM
neutrophil progenitors: 28 nCPM

Immune cell

eosinophil: 3.3 nTPM
neutrophil: 2.1 nTPM
classical monocyte: 0.5 nTPM
non-classical monocyte: 0.4 nTPM
basophil: 0.3 nTPM
intermediate monocyte: 0.2 nTPM

Brain region

cerebral cortex: 14 nTPM
thalamus: 14 nTPM
cerebellum: 12 nTPM
amygdala: 11 nTPM
basal ganglia: 11 nTPM
midbrain: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.43
gnomAD pLI: 0
gnomAD missense Z: 1.82
DepMap mean gene effect: -0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARAP3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

SH3KBP1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARAP3 as an antibody target. Whether an autoantibody or antibody against ARAP3 could matter depends on whether native ARAP3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARAP3 is annotated at the cell surface, where native ARAP3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ARAP3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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