Seroatlas · Human Serome Atlas

APLF

Aprataxin and PNK-like factor

Also known as: APLF_HUMAN, C2orf13, MGC47799, Xip1, ZCCHH1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8IW19
Gene
APLF
Ensembl
ENSG00000169621
Chromosome
2
Canonical length
511 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

Enables several functions, including ADP-D-ribose modification-dependent protein binding activity; nuclease activity; and poly-ADP-D-ribose binding activity. Involved in DNA repair; DNA repair-dependent chromatin remodeling; and protein localization to chromatin. Located in nucleoplasm. Is active in site of double-strand break. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

511 residues, UniProt reviewed canonical sequence.

>Q8IW19|APLF
     1  MSGGFELQPR DGGPRVALAP GETVIGRGPL LGITDKRVSR RHAILEVAGG QLRIKPIHTN
    61  PCFYQSSEKS QLLPLKPNLW CYLNPGDSFS LLVDKYIFRI LSIPSEVEMQ CTLRNSQVLD
   121  EDNILNETPK SPVINLPHET TGASQLEGST EIAKTQMTPT NSVSFLGENR DCNKQQPILA
   181  ERKRILPTWM LAEHLSDQNL SVPAISGGNV IQGSGKEEIC KDKSQLNTTQ QGRRQLISSG
   241  SSENTSAEQD TGEECKNTDQ EESTISSKEM PQSFSAITLS NTEMNNIKTN AQRNKLPIEE
   301  LGKVSKHKIA TKRTPHKEDE AMSCSENCSS AQGDSLQDES QGSHSESSSN PSNPETLHAK
   361  ATDSVLQGSE GNKVKRTSCM YGANCYRKNP VHFQHFSHPG DSDYGGVQIV GQDETDDRPE
   421  CPYGPSCYRK NPQHKIEYRH NTLPVRNVLD EDNDNVGQPN EYDLNDSFLD DEEEDYEPTD
   481  EDSDWEPGKE DEEKEDVEEL LKEAKRFMKR K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against APLF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.59
Highest tissue expression
2.2 nTPM

Expression across tissuesHPA

Tissue

  • skin: 2.2 nTPM
  • placenta: 1.7 nTPM
  • esophagus: 1.4 nTPM
  • liver: 1.4 nTPM
  • breast: 1.3 nTPM
  • vagina: 1.3 nTPM

Single-cell type

  • late spermatids: 352 nCPM
  • early spermatids: 281 nCPM
  • early primary spermatocytes: 75 nCPM
  • gonadotrophs: 72 nCPM
  • myonuclei: 66 nCPM
  • pituitary stem cells: 63 nCPM

Immune cell

  • basophil: 7.1 nTPM
  • eosinophil: 1.9 nTPM
  • T-reg: 1.4 nTPM
  • NK-cell: 1 nTPM
  • memory CD4 T-cell: 0.8 nTPM
  • myeloid DC: 0.8 nTPM

Brain region

  • basal ganglia: 11 nTPM
  • white matter: 10 nTPM
  • hippocampal formation: 9.5 nTPM
  • midbrain: 8.7 nTPM
  • cerebral cortex: 8.3 nTPM
  • amygdala: 8.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.53
gnomAD pLI
0
gnomAD missense Z
0.1
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of APLF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads APLF as an antibody target. Whether an autoantibody or antibody against APLF could matter depends on whether native APLF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

APLF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label APLF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/APLF. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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